不同类型的应激对血清实验指标的影响及其与HLA-DQB1等位基因的关联

发布时间：2017-12-30 19:38

本文关键词：不同类型的应激对血清实验指标的影响及其与HLA-DQB1等位基因的关联　出处：《大连医科大学》2008年硕士论文　论文类型：学位论文

【摘要】： 心理应激是由于个体在生活适应过程中,因环境要求与自身应付能力不平衡的认识所引起的一种紧张状态。应激主要由心理神经内分泌和免疫系统共同参与,通过下丘脑-垂体-肾上腺轴(HPA)和交感神经系统(SNS)调节免疫功能。机体免疫功能的紊乱造成机体免疫监视和免疫清除功能下降,使机体易发感染、自身免疫病和肿瘤等疾病。应激的研究目的是为人类的健康服务,选取人作为研究对象,采用天然的应激原使实验结果真实可信。因此选取不同类型的应激人群,检测其与正常对照人群的血清免疫、生化、凝血指标的变化,并结合其与HLA-DQB1等位基因的关系;动物模型的资源相对易得,可操作性强,因此建立束缚应激和热应激两种应激动物模型,检测其凝血功能的变化。共同探讨应激对机体的免疫功能的影响和其相关机制。方法 1.随机选取沈阳某重点中学(毕业班)学生27人,男生15人,女生12人,年龄18～20岁,高考前1个月采集血样,作为实验组;随机挑选某学校(入学新生)学生24人,男生12人,女生12人,年龄18～22岁,开学2个月后采集血样,作为对照组。 1.1研究考试应激对机体心理状态的影响,采用状态-特质焦虑量表和压力自评量表评估考试应激状态下机体的心理状态,评价应激状态下机体的心理反应。 1.2分别检测考试应激和对照组的血清生化、免疫指标,探讨应激对机体血液成分的影响。 2.随机挑选某地刑事拘留人员18人,(年龄43.28±11.04岁),男、女各半,刑拘7-15天后采血。对照组是随机挑选正常生活在社会生活中的群体成员24人,(年龄22.79±2.8岁),男、女各半。 2.1检测刑事拘留应激人员和对照组的血生化、免疫指标,探讨应激对机体血液成分的影响。 2.2检测刑事拘留应激人员和对照组的凝血指标,探讨应激对机体凝血系统的影响。 3.随机挑选大连医科大学本科、研究生10名,(年龄22-28岁),男、女各半,第一天正常睡眠,第二天晚上睡眠剥夺。 3.1分别检测正常睡眠后和睡眠剥夺后的血生化、免疫指标探讨应激对机体血液成分的影响。 3.2分别检测正常睡眠后和睡眠剥夺后的凝血指标,探讨应激对机体凝血系统的影响。 4.健康普通级昆明系(KM)小鼠,雌雄兼用,体重20-25g,应用束缚和热应激建立了两种小鼠应激动物模型和正常对照组。4.1分别检测三组小鼠的凝血指标,探讨应激对动物凝血系统的影响。 5.不同类型应激原所致的血清生化、免疫、凝血指标变化的比较。 6.采用PCR-SSP技术,建立HLA-DQB1等位基因的检测方法。对大学生HLA-DQB1等位基因频率进行检测。 7.综合得到的应激对机体生物学指标的影响及其与HLA-DQB1等位基因的关系,从基因角度探讨应激对机体的影响。结果 1.考试应激 1.1采用状态特质-焦虑问卷(STAI)和压力自评量表评估应激状态下机体的心理状态。确认高考前一个月的学生在焦虑状态和焦虑特质的得分均显著的高于全国的常模。而压力评分与常模无明显的差异。 1.2应考期与一般学习期(对照组)血清生化指标比较白蛋白(Alb)、白球比(a/g)、葡萄糖(Glu)、碱性磷酸酶(Alp)、钾(K)实验组较对照组显著升高。谷草转氨酶(AST)在应考期较一般学习期明显降低。应考期与一般学习期(对照组)血清免疫指标比较C3在应考期较一般学习期明显升高。 2.刑事拘留 2.1亚急性心理应激对刑事拘留人员会造成葡萄糖、钾离子、总胆固醇、高密度脂蛋白、低密度脂蛋白的显著升高;胰腺功能以及脂类可能是应激的敏感因子。 2.2亚急性心理应激对刑事拘留人员使IgA,C3,C4显著升高。 2.3亚急性应激对刑事拘留人员凝血指标的检测使凝血酶原形成时间缩短,而凝血酶时间延长。 3.睡眠剥夺 3.1睡眠剥夺使血清肌酐,总胆红素,直接胆红素显著降低,总蛋白显著升高。 3.2睡眠剥夺使凝血指标PT显著性降低。 4.应激组小鼠PT、APTT、TT值都较正常组延长,而两组不同应激的小鼠测定值无统计学显著差异。 5.高考应激和刑事拘留应激共同使葡萄糖、钾离子和补体C3水平升高;高考应激和睡眠剥夺应激共同使直接胆红素水平降低;刑事拘留应激和睡眠剥夺应激共同使PT水平下降。束缚应激和热应激在凝血指标上无明显差异。6.HLA-DQB1等位基因频率由高到低依次是:*03,*06,*05,*02,*04。 7.血清生化指标与HLA-DQB1等位基因的关联HLA-DQB1各等位基因阳性携带和阴性携带者各检测指标水平差异均无统计学意义。结论 1.高考,刑事拘留,睡眠剥夺均可以作为应激原而引起心理方面的改变,本文的研究人群可以作为应激的研究对象。 2.采用的束缚应激和热应激这两种应激是有效的应激动物模型。 3.不同类型的应激造成相应的生化、免疫、凝血指标的升高或降低有所不同。结果提示生化指标可以较客观的反应各器官的功能和躯体的健康程度。葡萄糖、钾离子、直接胆红素、补体C3、PT可为心理应激敏感因子。建立的小鼠心理应激和躯体应激的模型中,凝血因子不是躯体应激的相关性因子。APTT和TT是非特异性应激指标。
[Abstract]:Psychological stress is due to the individual life in the process of adaptation, because of a nervous state and their cognitive ability to cope with environmental requirements imbalance caused by psychological stress. Neuroendocrine and immune system, the hypothalamic pituitary adrenal axis (HPA) and sympathetic nervous system (SNS) to regulate the immune function. Immune dysfunction caused by immune surveillance and immune function decline, so that the body is prone to infection, autoimmune diseases and cancer and other diseases. The purpose of the study is to stress the human health services, selected as the research object, using natural stressor. The experiment result is true and credible. So choose different types of stressors the detection, determination of serum and normal controls biochemical changes of coagulation index, and its relationship with the HLA-DQB1 allele; animal model of resources that are easy to get, but Therefore, two stress animal models of restraint stress and heat stress were established to detect the changes of coagulation function, and to explore the influence of stress on the immune function of the body and its related mechanisms.
Method
1. randomly selected from a key middle school of Shenyang (graduating class) 27 students, 15 boys and 12 girls, age 18~20 years, 1 months before the college entrance examination blood samples were collected as experimental group; randomly selected a school (freshmen) 24 students, 12 boys and 12 girls, age 18~22 years, the school 2 months after the blood samples were collected as the control group.
1.1, we studied the influence of examination stress on the mental state of the body. We used state trait anxiety scale and stress self rating scale to assess the mental state of the body under the stress condition of examination, and evaluated the psychological response of the body under stress.
1.2 test the serum biochemical and immune indexes of the test stress and the control group, and discuss the effect of stress on the blood components of the body.
2., a total of 18 criminal detainees were selected at random (age 43.28 + 11.04 years). Male and female were half and arrested for 7-15 days. Blood samples were collected from the control group. The control group was randomly selected from 24 members of the group living in social life (age 22.79 to 2.8 years), male and female half.
2.1 the blood biochemical and immune indexes of the criminal detention stress personnel and the control group were detected, and the influence of stress on the blood components of the body was discussed.
2.2 the blood coagulation indexes of the stress personnel and the control group were detected and the effect of stress on the body's blood coagulation system was investigated.
3. randomly selected Dalian Medical University undergraduate, 10 graduate students (age 22-28 years old), male, female half, the first day normal sleep, second nights sleep deprivation.
3.1 the blood biochemistry after normal sleep and sleep deprivation was detected respectively, and the effects of stress on the blood components of the body were investigated.
3.2 the indexes of blood coagulation after normal sleep and sleep deprivation were detected respectively, and the effect of stress on the body's coagulation system was investigated.
4. healthy normal Kunming line (KM) mice, both male and female, weighing 20-25g. Two kinds of mouse stress animal models and normal control group were established by restraint and heat stress..4.1 was used to detect coagulation indexes of three groups of mice, and to explore the effect of stress on animal coagulation system.
5. the changes of serum biochemical, immune and coagulation indexes caused by different types of stress.
6. the detection method of HLA-DQB1 allele was established by PCR-SSP technique. The frequency of HLA-DQB1 allele in college students was detected.
7. the effects of stress on the biological indicators of the body and the relationship with the HLA-DQB1 allele, and the effect of stress on the body from the perspective of gene.
Result
1. test stress
1.1 using the State Trait Anxiety Inventory (STAI) and self rating scale to assess the psychological status of the body under stress. Confirm one month before the college entrance examination students in state anxiety and trait anxiety scores were significantly higher than the national norm. The stress score and the norm has no obvious difference.
1.2 exam and general learning period (control group) serum biochemical indicators of albumin (Alb), than the white ball (a/g), glucose (Glu), alkaline phosphatase (Alp), potassium (K) experimental group was significantly higher than that of control group (AST). AST in the exam than the general learning stage decreased should the exam. And general learning period (control group) serum immune index C3 in the exam should be more general learning period increased significantly.
2. criminal detention
2.1, subacute psychological stress can cause significant increases in glucose, potassium, total cholesterol, high-density lipoprotein and low density lipoprotein in criminal detainees, and pancreatic function and lipids may be sensitive factors of stress.
2.2 subacute psychological stress significantly increased IgA, C3, and C4 for criminal detainees.
2.3 the detection of coagulation indexes in criminal detainees caused by subacute stress shortened the time of prothrombin formation and the time of thrombin prolonged.
3. sleep deprivation
3.1 sleep deprivation significantly decreased serum creatinine, total bilirubin and direct bilirubin, and increased the total protein.
3.2 sleep deprivation reduced the significance of coagulation index PT.
The values of PT, APTT and TT in the 4. stress group were all longer than those in the normal group, but there was no significant difference between the two groups of mice with different stress.
5. college entrance examination stress and stress of criminal detention to make glucose, potassium and elevated levels of complement C3; college entrance examination stress and sleep deprivation stress reduced the direct bilirubin level of the common criminal detention; stress and sleep deprivation stress decreased the level of PT in common. Restraint stress and heat stress on blood coagulation index did not differ significantly between the.6.HLA-DQB1 allele frequency is high to low is: *03, *06, *05, *02, *04.
7. there was no significant difference in serum biochemical indexes and HLA-DQB1 alleles between HLA-DQB1 alleles positive carriers and negative carriers.
conclusion
1. the college entrance examination, criminal detention and sleep deprivation can all cause psychological changes as the source of stress. The research population in this paper can be used as the research object of stress.
2. the two kinds of stress, the restraint stress and the heat stress, are the effective animal models of stress.
3. different types of stress caused by the biochemical, immune, blood coagulation index increased or decreased in different degree. The results suggest that health biochemical indexes of each organ can be more objective response function and body. Glucose, potassium ion, direct bilirubin, complement C3, PT can be a sensitive factor. The mice heart should be stimulated and psychological stress the physical stress model, coagulation factor is not physical stress correlation factor.APTT and TT are non specificity index.

【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

【参考文献】