四味芳香开窍药抗急性脑缺血的实验研究及药性初探

发布时间：2017-12-31 05:03

本文关键词：四味芳香开窍药抗急性脑缺血的实验研究及药性初探　出处：《成都中医药大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的：平行观察四味芳香开窍药(麝香、冰片、苏合香、安息香)抗急性脑缺血的作用,并探讨其部分作用机制,同时试探急性脑缺血对模型动物心脏的影响,以期阐明该类药开窍醒神功效和“心—辛—浮”药性系统的科学内涵,为初步建立类“热闭证”动物模型提供一定实验依据。方法：①采用小鼠断头张口实验、饱和氯化镁致小鼠急性脑缺血实验及线栓法阻断大鼠大脑中动脉6h致大鼠局灶性脑缺血模型实验,通过观察小鼠存活时间、模型大鼠神经功能、脑梗死面积及脑组织形态学的变化,评价芳香开窍抗急性脑缺血的作用。②测定局灶性脑缺血模型大鼠脑内超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、Na+-K+-ATPase,Ca++-Mg++-ATPase的活力及血清一氧化氮(NO)含量,探讨芳香开窍药抗脑缺血的部分作用机制。③观察线栓法致大脑局灶性脑缺血模型大鼠心电图,心肌组织形态学和心SOD活性、MDA含量及Na+-K+-ATPase、Ca++-Mg++-ATPase活力的改变,试探脑缺血与心脏功能的相关性。④测定模型大鼠体温、心率与血压的变化,为复制类“热闭证”动物模型提供一定实验依据。结果：①以麝香、冰片为代表的芳香开窍药能增加断头后小鼠的张口次数与时间,能延长注射饱和氯化镁溶液后小鼠的张口喘气时间(P0.05)。②线栓法阻断大鼠大脑中动脉后,模型组大鼠苏醒时间延长(P0.05),眼口紧闭,病灶对侧前爪内收不能完全伸展,出现神经功能损伤(P0.05),并且体温明显升高(P0.05)。③与模型组比较,以麝香、冰片为代表的芳香开窍药能减轻线栓法致大脑局灶性脑缺血大鼠神经功能的损伤(P0.05),缩小大鼠局灶性脑缺血梗死面积并减轻其病理改变(P0.05)。④以麝香、冰片为代表的芳香开窍药能降低线栓法致大脑局灶性脑缺血大鼠脑内MDA含量(P0.05),提高脑内SOD活性(P0.05)；并且还能增加脑缺血模型大鼠脑内Na+-K+-ATPase, Ca++-Mg++-ATPase的活力(P0.05),但对血清NO含量无影响(P0.05)。⑤与假手术组比较,线栓法致大脑局灶性脑缺血6h大鼠心电图、心肌组织形态学未发生病理性改变,心肌SOD活性、MDA含量及Na+-K+-ATPase,Ca++-Mg++-ATPase的活力也无明显变化(P0.05)。结论：①以麝香、冰片为代表的芳香开窍药具有抗急性脑缺血的作用,其机制可能与降低脑内MDA含量、提高脑内SOD活性及增加脑缺血大鼠脑内Na+-K+-ATPase,Ca++-Mg++-ATPase的活力有关。②初步建立了类“热闭证”大鼠模型,四味芳香开窍药对模型动物体温、心率及血压影响的差异反映了该类药在“四性”上的不同。③芳香开窍药抗急性脑缺血作用是其开窍醒神功效的药效表征,也是其“辛—心—浮”药性系统的药效谱。④线栓法致大脑局灶性脑缺血6h的大鼠,未能发现心脏病理性的改变。
[Abstract]:Objective: to observe the effects of four kinds of aromatic orifices (musk, borneol, heparin and benzoin) on acute cerebral ischemia in parallel, and to explore the effects of acute cerebral ischemia on the heart of model animals. The purpose of this paper is to clarify the scientific connotation of this kind of medicine and to provide some experimental basis for the establishment of the animal model of "heat closing syndrome". Methods the mouse head opening test, the acute cerebral ischemia induced by saturated magnesium chloride and the focal cerebral ischemia model of rats by blocking the middle cerebral artery for 6 hours were used. The changes of survival time, neural function, cerebral infarction area and brain histomorphology of the model rats were observed. To evaluate the effect of aromatics on acute cerebral ischemia. 2 to measure the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the brain of rats with focal cerebral ischemia. The activity of Ca -Mg ATPase and the content of nitric oxide (no) in serum of Na K ATPase. Objective: to investigate the mechanism of anti-cerebral ischemia by aromatic resuscitation drugs. 3 observe the electrocardiogram (ECG), myocardial morphology and cardiac SOD activity in rats with focal cerebral ischemia induced by thread embolism. The changes of MDA content and Na-K-ATPase activity of Ca-mg-ATPase were studied. The correlation between cerebral ischemia and cardiac function was explored. 4. 4. The temperature of the model rats was measured. The changes of heart rate and blood pressure provide some experimental basis for duplicating the animal model of heat closure syndrome. Results the aromatic orifices represented by musk and borneol could increase the opening times and time of severed mice. After blocking the middle cerebral artery (MCA) of rats by P0.052.Then prolonging the gasping time of mice injected with saturated magnesium chloride solution, the recovery time of rats in the model group was prolonged (P0.05) and the eyes were closed. The adducts of the contralateral anterior claws could not be completely extended, the nerve function was injured and the body temperature was significantly increased compared with the model group. Borneol as the representative aromatic resuscitation medicine can reduce the damage of neural function in rats with focal cerebral ischemia induced by thread embolization (P0.05). The infarct area of focal cerebral ischemia in rats was reduced and its pathological changes were alleviated (P0.05.4) with musk. Borneol as the representative aromatic resuscitation medicine can reduce the content of MDA and increase the activity of SOD in brain of rats with focal cerebral ischemia. It also increased the activity of Na-K-ATPase and Ca-mg-ATPase in the brain of cerebral ischemia model rats (P0.05). Compared with the sham operation group, the electrocardiogram (ECG) of the rats with focal cerebral ischemia induced by thread embolization did not show pathological changes in myocardial histomorphology. The content of SOD and the activity of Ca -Mg -ATPase in Na-K-AT Paseate also had no significant change (P 0. 05). Conclusion the aromatic orifices represented by musk and borneol have the effect of anti-acute cerebral ischemia, and the mechanism may be related to the decrease of MDA content in the brain. The activity of SOD in brain and Na -K ATPase in brain of ischemic rats were increased. The activity of Ca-mg-ATPase was related to the establishment of the rat model of "heat blocking syndrome", and the body temperature of the model animal was treated by four kinds of aromatic orifices. The difference of heart rate and blood pressure reflects that the anti-acute cerebral ischemia effect of three kinds of aromatic resuscitation drugs on "four properties" is a sign of the effect of resuscitation. It was also found that the changes of heart disease were not found in the rats with focal cerebral ischemia induced by the pharmacodynamic spectrum of the "Xin-Xin-Fu" drug system for 6 h.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R285.5;R-332

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