运动及心理应激对大鼠不同部位SOD活性的影响

发布时间：2018-01-01 19:26

本文关键词：运动及心理应激对大鼠不同部位SOD活性的影响　出处：《吉林大学》2009年硕士论文　论文类型：学位论文

【摘要】： 有关氧自由基的理论一直是运动医学领域研究的热点问题。所谓氧自由基,就是当机体进行代谢时,能夺去氧的一个电子,这个氧原子就变成自由基。自由基作为人体垃圾,能够促使某些疾病的发生和机体的衰老。超氧化歧化酶又称过氧化物歧化酶(Superoxide Dismutase, SOD) ,这种活性酶能专一地清除体内有害的自由基,以解除自由基氧化体内的某些组成成分而造成的机体损害。大量现代医学研究证明,SOD是人体内的一种最重要的细胞保护酶,既能防辐射损伤,又能有效地抵抗过氧化阴离子自由基,从而延缓衰老,调节机体代谢能力,提高人体自身的免疫功能。但是,究竟何种运动强度会使机体在运动后抗氧化能力增强,还有待于进一步研究。本实验通过对大鼠进行不同强度的运动训练及心理应激干预,探讨不同的运动负荷及心理应激对大鼠机体内SOD的抗氧化能力的影响。实验采用游泳方式对体重为350g-400g的大鼠进行中等强度(每天训练30min)、大强度(每天训练60min)、大强度心理应激(每天在容器内进行刺激后运动60min)的运动训练,训练8周后处死。取大鼠腹腔动脉血,离心后提取血清,肝脏、肾脏、心脏组织制取组织匀浆,采用比色法(试剂盒)测定各组大鼠血清及组织样本中的SOD。结果表明:不同运动强度和心理应激对大鼠机体抗氧化能力产生不同的影响。与对照组比较,中等强度、大强度、大强度运动加心理应激干预使机体的抗氧化能力均提高;但在不同组织内的SOD水平变化不同。
[Abstract]:The oxygen free radical theory is always a hot topic in the field of sports medicine. The oxygen free radical, is when the body for metabolism, oxygen can take an electron, the oxygen atoms become free radicals. The free radicals as human waste, can promote the occurrence of certain diseases and the aging body. Superoxide dismutase and superoxide dismutase (Superoxide Dismutase, SOD), the enzyme activity can only remove the body of harmful free radicals, the damage to remove certain components of the body caused by free radical oxidation. Studies show that lots of modern medicine, SOD is one of the most important protective enzyme in human body the both radiation damage, and can effectively resist the superoxide anion radical, thus delaying senescence, regulating body metabolism, improve the body's own immune function. However, what kind of strength will make the machine movement Further study is needed to improve the antioxidant capacity of the body after exercise. In this experiment, rats were subjected to different intensity of exercise training and psychological stress intervention to explore the effects of different exercise loads and psychological stress on the antioxidant capacity of SOD in rats.
The swimming on body weight of 350g-400g rats with moderate intensity (training day 30min), high intensity (training day 60min), high intensity and psychological stress (exercise stimulation in the container every day after 60min) training, training 8 weeks later. The rats abdominal cavity blood after centrifugal extraction serum, liver, kidney, heart tissue homogenate preparation, using colorimetric method (Kit) for determination of serum and tissue samples of SOD. rats
The results show that the different intensity of exercise and psychological stress have different effects on antioxidant capacity in rats. Compared with control group, moderate intensity, high intensity, high intensity exercise intervention psychological stress increases the body's antioxidant capacity were improved; but in different tissues SOD level changes are different.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R363

【参考文献】