重复磁刺激右侧大脑中动脉闭塞模型大鼠脑梗死区微环境及神经功能的变化

发布时间：2018-01-08 09:27

本文关键词：重复磁刺激右侧大脑中动脉闭塞模型大鼠脑梗死区微环境及神经功能的变化　出处：《中国组织工程研究》2015年27期 　论文类型：期刊论文

【摘要】：背景:国内外大量研究表明重复经颅磁刺激可使皮质兴奋性产生较刺激时间更加持久的改变,为磁刺激应用于脑梗死后康复治疗提供了一个新的研究方向,但其远期临床疗效与安全性尚需进一步研究。目的:观察重复经颅磁刺激脑梗死大鼠对神经再生微环境及功能恢复的影响。方法:将大鼠随机分为模型组、假刺激组及重复经颅磁刺激组(80%运动阈值(MT)组、100%MT组和120%MT组),采用线栓法制备大鼠右侧大脑中动脉闭塞模型。制模24 h后各重复经颅磁刺激亚组给予20 Hz相应强度磁刺激,假刺激组则给予假磁刺激,模型组制模后未给予特殊处理。结果与结论:造模后7 d,重复经颅磁刺激组的脑梗死体积显著小于模型组及假刺激组(P0.05)。RT-PCR、Western blot检测显示,造模后72 h,重复经颅磁刺激组水通道蛋白4/9基因和蛋白表达均较模型组显著增高(P0.05)。与造模后第1天比较,造模后第15天重复经颅磁刺激组(100%MT)神经功能缺损评分得到明显改善(P0.05)。免疫组织化学检测结果显示,各重复经颅磁刺激亚组缺血半暗带区胶质纤维酸性蛋白表达与模型组比较均显著减少(P0.05)。结果证实,重复经颅磁刺激可减轻脑梗死模型大鼠神经功能缺损程度,通过诱导脑缺血耐受、减少神经细胞凋亡和降低水通道蛋白4/9基因和蛋白的表达,改善神经再生微环境。
[Abstract]:Background: a large number of domestic and foreign research shows that repetitive transcranial magnetic stimulation can produce a stimulation of cortical excitability time more lasting change for magnetic stimulation for cerebral infarction after rehabilitation treatment provides a new research direction, but its long-term efficacy and safety still needs further study. Objective: To observe the effect of repetitive transcranial magnetic effect of stimulation of cerebral infarction rats on the microenvironment of the nerve regeneration and functional recovery. Methods: the rats were randomly divided into model group, sham stimulation group and repetitive transcranial magnetic stimulation group (80% motor threshold (MT) group, 100%MT group and 120%MT group), a rat model of middle cerebral artery occlusion molding model. After 24 h the repetitive transcranial magnetic stimulation sub group were given 20 Hz corresponding magnetic stimulation, sham stimulation group received sham TMS, HIBD model group was not given special treatment. Results and conclusion: after modeling 7 d repetitive transcranial magnetic stimulation group of brain The infarct volume was significantly smaller than that in the model group and the sham stimulation group (P0.05).RT-PCR display Western blot detection, 72 h after modeling, repetitive transcranial magnetic stimulation group of water channel protein 4/9 gene and protein expression were significantly higher than those in the model group (P0.05). Compared with the model after first days, fifteenth days after modeling, repeat transcranial magnetic stimulation group (100%MT) neurological function improved significantly (P0.05). The results of immunohistochemistry showed that the repetitive transcranial magnetic stimulation group ischemic penumbra glial fibrillary acidic protein compared with the model group were significantly reduced (P0.05). The results confirmed that can reduce the degree of neural function defect rat model of cerebral infarction by repetitive transcranial magnetic stimulation, through the induction of brain ischemic tolerance, and reducing the expression of water channel protein 4/9 gene and protein in the apoptosis of nerve cells, improve the microenvironment for nerve regeneration.

【作者单位】：天津医科大学总医院急诊科;天津医科大学总医院神经外科;
【分类号】：R743.3;R-332
【正文快照】： 0引言Introduction这些就是脑功能重组的结果。脑梗死为脑供血功能障碍而引起的缺血、缺氧性脑组重复经颅磁刺激(repetitive transcranial magnetic织坏死和软化[1-3]。具有高发病率、高致残率、高死亡率和stimulation,rT MS)可使皮质兴奋性产生较刺激时间更加持高复发率的特

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