小鼠粪便及肠道各部位内容物细菌群落结构差异分析

发布时间：2018-01-08 09:38

本文关键词：小鼠粪便及肠道各部位内容物细菌群落结构差异分析　出处：《中国实验动物学报》2015年03期 　论文类型：期刊论文

【摘要】：目的分析小鼠粪便及肠道各部位内容物细菌群落结构差异,探讨粪便取样研究肠道微生物与疾病关系的科学性和客观性,为相关实验设计提供参考。方法利用末端限制性片段长度多态性(T-RFLP)、变性梯度凝胶电泳(DGGE)和荧光定量PCR(q PCR)技术对BALB/c小鼠肠道不同部位(十二指肠、空肠、回肠、盲肠、结肠和直肠)内容物及粪便中细菌群落组成和丰度进行比较,分析细菌群落在小鼠粪便及肠道各部位的分布差异。结果粪便与直肠、结肠中优势片段均为244、255 bp和449 bp,而小肠内容物(十二指肠、空肠及回肠)的优势片段则为60、73、261、268 bp和272 bp,且小肠各部位之间细菌群落结构差异较大;十二指肠和空肠内容物中的细菌丰度较低,分别为6.9 log(copies)/g和8.3 log(copies)/g,而粪便中细菌丰度高达11.8 log(copies)/g,约2倍于十二指肠细菌丰度,显著高于十二指肠和空肠(P0.05),与大肠各部位及回肠内容物细菌丰度相当,差异不显著(P0.05)。结论大肠各部位内容物和粪便的细菌群落结构相似,个体差异较小肠小,适合通过粪便取样进行大肠部位特别是结直肠的肠道微生态与某些疾病的相关分析。
[Abstract]:Objective to analyze the differences of bacterial community structure in feces and various parts of intestinal tract of mice, and to explore the scientific and objective nature of stool sampling to study the relationship between intestinal microbes and diseases. Methods the terminal restriction fragment length polymorphism (TRFLP) was used. Denaturing gradient gel electrophoresis (DGGE) and fluorescence quantitative PCR(q PCR were used to study the different parts of intestine (duodenum, jejunum, ileum, cecum) in BALB/c mice. The composition and abundance of bacterial community in colon and rectum) contents and feces were compared and the distribution differences of bacterial communities in feces and intestines of mice were analyzed. The dominant fragments in colon were 244,255 BP and 449 BP, while the dominant fragments in small intestinal contents (duodenum, jejunum and ileum) were 6073p261. 268 BP and 272 BP, and there were significant differences in bacterial community structure among different parts of the small intestine. The bacterial abundance in duodenum and jejunum was 6.9 log(copies)/g and 8.3 log(copies)/g, respectively. The bacterial abundance in feces was 11.8 log-copiesl / g, about 2 times higher than that in duodenum, and significantly higher than that in duodenum and jejunum (P0.05). The bacterial abundance was similar to that of the large intestine and ileum contents, but the difference was not significant (P 0.05). Conclusion the bacterial community structure of the large intestine contents and feces is similar, and the individual difference is smaller than that of the small intestine. It is suitable to analyze the relationship between intestinal microecology and some diseases in large intestine, especially in colorectal.
【作者单位】：新乡医学院基础医学院;新乡医学院第一附属医院;
【基金】：国家自然科学基金(40901125,81201765) 新乡医学院科研培育基金(2013ZD102)
【分类号】：R37
【正文快照】： 。studies on the relationship between large intestine especially colorectal microbiota and diseases may be conducted via fecalsampling.肠道中含有数量巨大的微生物,数量超过1014,约10倍于人体细胞[1]。这些微生物在维持人类生命健康方面发挥着重要作用[2]。近来研

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