中国MERS输入病例病毒基因组测序及分析

发布时间：2018-01-08 13:40

本文关键词：中国MERS输入病例病毒基因组测序及分析　出处：《中国科学:生命科学》2015年08期 　论文类型：期刊论文

【摘要】：为测定分析中国第一例输入性中东呼吸综合征冠状病毒基因组序列.采用PCR分段扩增、测序、拼接的方法,组装病毒基因组序列,应用BLAST,MEGA6.0,SIMPLOT,RDP等软件分析病毒序列的相似性、变异、进化、重组事件.结果发现,所完成的病毒序列长度为29928 bp,与韩国报道的序列最为接近.病毒核苷酸序列与近期中东地区分离到的病毒株序列相似性高达99.53%~99.92%;在多聚蛋白ORF1ab中有3个新发突变,而S,E,M,N蛋白未出现变异;核苷酸序列无明显的片段重组现象.本研究表明,中国广东第一例输入性MERS患者的序列没有出现明显的变异,一些位点突变后功能变化仍需进一步研究.
[Abstract]:In order to determine and analyze the genome sequence of the first imported Middle East Respiratory Syndrome coronavirus from China, the genome sequence of the virus was assembled by PCR fragment amplification, sequencing and splicing, and BLAST was used. MEGA 6.0 / simple RDP software was used to analyze the similarity, variation, evolution and recombination of virus sequence. The result showed that the length of virus sequence was 29928 BP. The nucleotide sequence of the virus is similar to that of the virus strain isolated recently in the Middle East, and the similarity of the nucleotide sequence of the virus is 99.53 and 99.92 respectively. There were three new mutations in the polymorphic protein ORF1ab, but no mutation in the protein. There was no significant fragment recombination in nucleotide sequence. This study showed that there was no significant variation in the sequence of the first imported MERS patient in Guangdong, China, and the functional changes after some locus mutations still need to be further studied.
【作者单位】：南方医科大学公共卫生与热带医学学院;广东省疾病预防控制中心微生物检验所;
【基金】：NFSC-广东省联合基金(批准号:U1132002) 广东省科技计划项目(批准号:2010A40302003) 国家自然科学基金(批准号:81272483)资助
【分类号】：R373.1
【正文快照】： 引用格式:谢茜,曹宇娟,苏娟,等.中国MERS输入病例病毒基因组测序及分析.中国科学:生命科学,2015,45:799 803自2012年9月第一例中东呼吸综合征(MiddleEast Respiratory Syndrome,MERS)患者确诊以来[1,2],该病波及23个国家,死亡率达近40%[3],成为全球威胁人类健康的重要疾病.近

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