转基因番茄可食防龋疫苗免疫大鼠的实验研究

发布时间：2018-01-14 15:02

本文关键词：转基因番茄可食防龋疫苗免疫大鼠的实验研究　出处：《重庆医学》2015年23期 　论文类型：期刊论文

【摘要】：目的用表达变异链球菌表面蛋白PAcP和霍乱毒素B亚单位融合蛋白的转基因番茄免疫SD大鼠,检测其免疫原性,探索研制安全、有效的可食用防龋疫苗的可能性。方法选择雌性SD大鼠18只,建立龋齿模型,随机分成3组(n=6),分别为转基因番茄组(实验组)、变异链球菌灭活全菌免疫组(阳性对照组)、非转基因番茄组(阴性对照组),免疫方式为灌胃免疫,每周免疫1次,连续免疫4周。分别于首次免疫前1d和每次免疫1周后采集血液、唾液样品,用酶联免疫吸附实验法检测血清中免疫球蛋白G(IgG)、唾液中分泌型免疫球蛋白A(SIgA)抗体水平;鼠龄70d时处死动物,并取上下颌骨进行龋齿计分。结果免疫后,实验组和阳性对照组大鼠血清中IgG、唾液中SIgA抗体水平与阴性对照组比较差异有统计学意义(P0.05);实验组和阴性对照组在Dx级外的各级差异有统计学意义(P0.05)。结论转基因番茄防龋疫苗具有免疫原性,能够诱导实验动物产生有效的免疫应答,降低龋齿的发生。
[Abstract]:Objective to immunize SD rats with transgenic tomato expressing Streptococcus mutans surface protein PAcP and cholera toxin B subunit fusion protein. Methods 18 female SD rats were selected to establish dental caries model and were randomly divided into 3 groups: transgenic tomato group (experimental group). Streptococcus mutans inactivated whole bacteria immunization group (positive control group, non-transgenic tomato group (negative control group), the way of immunization was intragastric immunization, once a week. The blood and saliva samples were collected 1 day before the first immunization and 1 week after each immunization. The serum immunoglobulin G G G g was detected by enzyme linked immunosorbent assay (Elisa). The level of secretory immunoglobulin A Siga antibody in saliva; The animals were killed at 70 days of age and the upper mandible was taken to score the dental caries. Results after immunization, IgG in serum of the experimental group and the positive control group was obtained. The level of SIgA antibody in saliva was significantly higher than that in negative control group (P 0.05). The difference between the experimental group and the negative control group was statistically significant (P 0.05). Conclusion transgenic tomato anti-caries vaccine has immunogenicity and can induce an effective immune response in experimental animals. Reduce the incidence of dental caries.
【作者单位】：遵义医学院口腔学院;贵州省高等学校口腔疾病研究特色重点实验室;贵阳市口腔医院口腔内科;辽河油田总医院口腔科;
【基金】：国家自然科学基金资助项目(30160086) 贵州省第6批科技创新团队资助项目[黔科合人才团队(2013)4026号] 贵州省高等学校重点学科建设项目(SZXK-201207-04)
【分类号】：R392
【正文快照】： 龋病是人类最常见的细菌感染性疾病之一。就病因而言,细菌是龋病发病的主要因素,变异链球菌(S.mutans)表面蛋白PAc是被认为重要的毒力因子。PAc的P区是脯氨酸富集区,P区多肽片段中含有B细胞表位,具有免疫原性和免疫反应性[1]。防龋疫苗经历了包括细菌活载体疫苗[2]、亚单位疫

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