参麦注射液对术前阿霉素化疗乳腺癌裸鼠模型心肌保护作用的研究

发布时间：2018-01-14 20:21

本文关键词：参麦注射液对术前阿霉素化疗乳腺癌裸鼠模型心肌保护作用的研究　出处：《广西医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：研究参麦注射液(SMI)干预治疗对ADM化疗的乳腺癌裸鼠模型在围术期心肌保护的作用及可能机制。方法：种植乳腺癌细胞MCF-7于裸鼠乳房垫,建立乳腺癌裸鼠模型。将制作成功的乳腺癌裸鼠27只,随机分成3组：A组(生理盐水组)、B组(ADM+生理盐水组)、C组(ADM+SMI组)。在T1(干预前)、T2(麻醉前)、T3(麻醉后20min)三个时点分别取血检测血清cTnI水平、SOD活力和MDA含量。麻醉后取心肌组织检测NRF-1的表达水平、腺苷酸池的含量及光、电镜下观察结构改变。结果：(1)T1、T2时刻,B、c组的cTnI均显著高于A组(P0.01)；T3时刻,A、C组的cTnI显著低于B组(P0.01；P0.05),A组低于C组(P＜0.01)。B组在T2及T3时刻的cTnI均显著高于T1时刻(P0.05)。(2)T1时刻,三组之间的SOD活力无统计差异(P＞0.05)；T2时刻,B、C组SOD均显著低于A组(P＜0.01),B组低于C组(P0.05)；T3时刻,A、C组的SOD活力均显著高于B组(P0.01；P0.05),A组高于C组(P0.01)。B组SOD在T2及T3时刻均显著低于T1时刻(P0.01)。(3)三个时刻,B、C组的MDA含量均显著高于A组(P0.05)；C组的MDA在T1时刻显著高于T2及T3时刻(P0.05)。(4)A、C组NRF-1mRNA的表达均显著高于B组(P0.05),A、C组对比无统计学差异(P0.05)。(5)A、C组的ATP含量均显著高于B组(P0.05)；B组的AMP含量均显著高于A、C组(P0.05)；A组腺苷酸代谢池总量高于B组(P0.05)。(6)C组的病理评分显著低于B组(P0.05),但仍然高于A组(P0.05)。(7)与A组比较,B、C组的心肌组织超微结构均发生严重改变,但C组的改变较轻。结论：(1)ADM化疗乳腺癌裸鼠模型可引起心肌损伤,SMI的干预治疗可减轻心肌的再次受损；(2)SMI的干预治疗降低了裸鼠机体的脂质过氧化反应、增强了抗氧化能力,减少心肌线粒体的损伤；(3)SMI降低了ADM对核基因NRF-1表达的抑制作用、增加腺苷酸池中高能磷酸化合物含量,保护了心肌能量代谢途径。
[Abstract]:Aim: to study the protective effect and possible mechanism of Shenmai injection on myocardial protection in nude mice model of breast cancer treated with ADM chemotherapy during perioperative period. Methods: breast cancer cell line MCF-7 was implanted into breast pad of nude mice to establish nude mouse model of breast cancer. 27 nude mice were randomly divided into 3 groups: group A (normal saline group). Group B was treated with normal saline group and group C was treated with SMI. Blood samples were collected at 20 min after anesthesia to detect the activity of cTnI and the content of MDA in serum. The expression of NRF-1 was detected in myocardium after anesthesia. The content and light of adenylate cell were observed under electron microscope. Results the cTnI of T1T 2 group was significantly higher than that of A group (P 0.01). The cTnI of group C was significantly lower than that of group B (P 0.01). The cTnI of group A was lower than that of group C (P < 0.01). The cTnI of group B at T _ 2 and T _ 3 was significantly higher than that of group A at T _ 1 and T _ 1. There was no statistical difference in SOD activity among the three groups (P > 0.05). The SOD of group C was significantly lower than that of group A (P < 0.01) and group B was lower than that of group C (P < 0.05). The activity of SOD in group T 3 was significantly higher than that in group B (P 0.01). The SOD of group A was significantly lower than that of group C at T _ 2 and T _ 3. The content of MDA in group C was significantly higher than that in group A (P 0.05). The expression of MDA in group C was significantly higher than that in group C at T1 and T3, respectively. The expression of NRF-1mRNA in group C was significantly higher than that in group B (P0.05A). The ATP content of group C was significantly higher than that of group B (P 0.05). The content of AMP in group B was significantly higher than that in group A (P 0.05). The total amount of adenylate metabolism pool in group A was significantly lower than that in group B (P 0.05), but still higher than that in group A (P 0.05). The ultrastructure of myocardial tissue in group C was significantly changed, but the change in group C was mild. Conclusion the nude mice model of chemotherapy with ADM can induce myocardial injury and the intervention of SMI can reduce the myocardial redamage. The intervention of SMI decreased lipid peroxidation, enhanced antioxidant capacity and reduced myocardial mitochondria damage in nude mice. SMI decreased the inhibitory effect of ADM on the expression of nuclear gene NRF-1, increased the content of high-energy phosphates in adenylic acid pool, and protected myocardial energy metabolism pathway.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R737.9;R-332

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