兔抗戊型肝炎病毒抗体定量线性标准品的标定及初步应用

发布时间：2018-01-16 13:14

本文关键词：兔抗戊型肝炎病毒抗体定量线性标准品的标定及初步应用　出处：《中国病毒病杂志》2015年02期 　论文类型：期刊论文

【摘要】：目的建立兔抗戊型肝炎病毒(hepatitis E virus,HEV)抗体定量线性标准品并对其应用效果进行评价。方法利用抗-HEV抗体检测试剂盒筛选出本实验室的抗-HEV抗体阳性兔血清S26。用世界卫生组织(World Health Organization,WHO)抗-HEV Ig标准品对其进行标定并检测其稳定性。将S26血清稀释至其线性范围,再倍比系列稀释5个稀释度作为定量线性标准,进行多次实验考评其重复性和应用效果。结果经3次标定,S26血清抗-HEV水平为39.54IU/ml,95%CI为39.47~39.62IU/ml,变异系数0.1%,稳定性好。从3.95IU/ml开始的5个倍比系列稀释度可作为兔抗-HEV抗体定量线性标准品,其重复性好且可有效地反映兔血清抗体水平变化趋势。结论本研究建立的兔抗-HEV抗体定量线性标准品,稳定性、重复性好,可用于兔HEV的疫苗原性评价及流行病学调查。
[Abstract]:Objective to establish rabbit anti-hepatitis E virus. HEV). Methods the anti-HEV antibody positive rabbit serum S26 was screened by using anti-HEV antibody detection kit. World Health Organization. WHO) anti-HEV Ig standard was calibrated and its stability was determined. The serum S26 was diluted to its linear range, and the dilution of the series of double times was used as the quantitative linear standard. Results the serum anti-HEV level of S26 was 39.54 IU / ml after three times calibration. 95 CI was 39.47 ~ 39.62 IUs / ml, coefficient of variation was 0.1%. The series dilution of 5-fold ratio starting from 3.95 U / ml can be used as a quantitative linear standard for rabbit anti-HEV antibody. Conclusion the quantitative linear standard of rabbit anti-HEV antibody established in this study is stable and reproducible. It can be used to evaluate the immunogenicity of rabbit HEV and to investigate its epidemiology.
【作者单位】：北京大学医学部病原生物学系和感染病中心;
【基金】：国家自然科学基金(81271827) 高等学校博士学科点专项科研基金(20120001110098)
【分类号】：R392-33
【正文快照】： 戊型肝炎病毒(hepatitis E virus,HEV)是急性病毒性肝炎的病原体之一。研究表明,抗-HEV抗体对HEV感染具有一定的保护性[1]。然而,以往的实验检测抗-HEV抗体均采用定性或半定量的方法,其结果不能准确地反映抗体定量水平与保护性的关系。为了准确评价疫苗免疫后以及HEV感染过程

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