SLC34A2基因真核表达载体的构建及其转染人肺泡上皮细胞的实验研究

发布时间：2018-01-20 15:53

本文关键词： SLC34A2基因肺泡上皮细胞钙、磷肺泡微石症　出处：《吉林大学》2008年博士论文　论文类型：学位论文

【摘要】： SLC34A2是编码磷酸钠协同转运蛋白的基因,主要参与无机磷代谢,表达在人体的多种组织中,以肺组织表达最高。本研究①从正常人肺组织中获取了该基因完整的cDNA,构建了真核表达载体;②然后将该真核表达载体用脂质体的方法成功转染人肺泡上皮细胞,观察在过表达的情况下和不同药物刺激下,细胞外液中钙、磷的变化;③并在国内首次对基因SLC34A2的7个外显子进行了测序。④本研究第一次成功克隆了SLC34A2基因,并转染肺泡上皮细胞;⑤观察了不同情况下,该基因对细胞外液钙、磷转运的影响;⑥同时我们在临床上跟踪、治疗一肺泡微石症家系,获得了肺泡微石症的第一手临床资料。本研究填补了国内对SLC34A2基因研究的空白,是肺泡微石症等微小结石类疾病发病机制的又一个途径,为治疗这类疾病提供了可参考的理论价值,为更深入探讨SLC34A2基因的功能和信号转导机制奠定了重要的实验基础。
[Abstract]:SLC34A2 is a gene encoding sodium phosphate cotransporter. It is mainly involved in inorganic phosphorus metabolism and is expressed in a variety of human tissues. In this study, complete cDNAs were obtained from normal human lung tissue and eukaryotic expression vector was constructed. (2) the eukaryotic expression vector was successfully transfected into human alveolar epithelial cells by liposome, and the changes of calcium and phosphorus in extracellular fluid were observed under the condition of overexpression and under the stimulation of different drugs. (3) the 7 exons of gene SLC34A2 were sequenced for the first time in our country. In this study, SLC34A2 gene was cloned successfully and transfected into alveolar epithelial cells for the first time. (5) the effects of the gene on the transport of calcium and phosphorus in extracellular fluid were observed under different conditions. At the same time, we followed up and treated a pedigree with alveolar microlithiasis, and obtained first-hand clinical data of alveolar microlithiasis. This study filled the blank of SLC34A2 gene research in China. It is another way of pathogenesis of microlithiasis such as alveolar microlithiasis, which provides a theoretical value for the treatment of these diseases. It lays an important experimental foundation for further study on the function and signal transduction mechanism of SLC34A2 gene.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2008
【分类号】：R346

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