SENP3对不同程度氧化应激的感受和应答

发布时间：2018-01-22 01:00

本文关键词： SENP 氧化应激抗氧化酶基因表达　出处：《中国细胞生物学学报》2015年07期 　论文类型：期刊论文

【摘要】：细胞在受到内源、外源的氧化物刺激时均能启动应答机制,改变蛋白质的量、定位和活性来抵抗应激。作者前期的研究发现,SUMO特异蛋白酶SENP3(Sentrin/SUMO specific protease3)可以感受一定程度的氧化应激而发生量的累积,从而影响一系列转录因子的SUMO化修饰状态和特异基因表达,发挥应答应激的功能,但在不同程度氧化应激时SENP3是否有不同的感受和应答机制并不清楚。该研究应用不同剂量H2O2模拟不同程度氧化应激,探讨SENP3的量、定位改变和对抗氧化蛋白表达的影响。结果显示,轻度氧化应激即可造成SENP3量的增加,但无H2O2剂量相关关系,而不同程度氧化应激均可引起SENP3从核仁向核质的移位,且随H2O2剂量增加而增加。在氧化应激引起的过氧化物氧还蛋白4(peroxiredoxin 4,Prx4)、超氧化物歧化酶1(superoxide dismutase1,SOD1)及过氧化氢酶(catalase,CAT)表达上调中,SENP3介导了这种表达改变,且有H2O2剂量相关性。该研究一方面发现了SENP3感受不同程度氧化应激的两种机制,另一方面也发现了SENP3介导抗氧化应答的功能,提示SENP3在细胞精细的应激应答机制中扮演了重要角色,具有一定的生理和病理意义。
[Abstract]:Cells in response to endogenous, can start response mechanism of exogenous oxide stimulation, change the protein quantity, location and activity to resist stress. The research found that SUMO (Sentrin/SUMO specific protease3 SENP3 specific protease) can accumulate experience oxidative stress to a certain extent and quantity, thus affecting the expression of SUMO modification a series of specific genes and transcription factors, play the function of stress response, but SENP3 in different levels of oxidative stress have different feelings and response mechanism is not clear. The research and application of different doses of H2O2 to simulate different degree of oxidative stress, the amount of SENP3, and change the expression of anti oxidative protein localization. The results showed that stress can cause mild oxidation of the increase of SENP3 content, but no correlation between the dose of H2O2, and different degree of oxidative stress can cause the SENP3 from the nucleolus to cytoplasm The shift, and increased with the doses of H2O2. Oxygen peroxide to oxidative stress caused by protein 4 (peroxiredoxin 4, Prx4), superoxide dismutase (superoxide dismutase1, 1 SOD1) and catalase (catalase, CAT) expression, SENP3 mediated the expression of H2O2 and the dose related. On the one hand found SENP3 feel the two mechanism of different degree of oxidative stress, on the other hand also found that SENP3 mediated antioxidant response function, suggesting that SENP3 in the mechanism of the stress responsive cells fine plays an important role, with the physiological and pathological significance.

【作者单位】：上海交通大学医学院生物化学与分子细胞生物学系上海肿瘤微环境和炎症重点实验室;
【基金】：上海市浦江人才计划(批准号:2013D024) 国家自然基金重点项目(批准号:31230037) 科技部973子项目(批准号:2013CB910902)资助的课题~~
【分类号】：R363
【正文快照】： 上海市浦江人才计划(批准号:2013D024)、国家自然基金重点项目(批准号:31230037)和科技部973子项目(批准号:2013CB910902)资助的课题网络出版时间:2015-07-06 13:57 URL:http://www.cnki.net/kcms/detail/31.2035.Q.20150706.1357.002.html正常情况下,细胞内存在适中的活性氧(

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