放射性白内障动物模型的建立及相关指标的检测

发布时间：2018-01-25 21:19

  本文关键词： X-射线 放射性白内障 αB-晶体蛋白　出处：《苏州大学》2009年硕士论文　论文类型：学位论文

【摘要】： 目的:利用动物实验,通过X射线照射S-D大鼠双眼建立放射性白内障动物模型,观察X射线剂量与白内障严重程度之间的关系;检测受不同剂量X射线照射的大鼠晶状体中可溶性αB-晶体蛋白的表达水平,探讨αB-晶体蛋白与放射性白内障形成之间的关系。 方法:照射前选取8只大鼠作为正常对照组,实验照射组大鼠24只,平均分成3组每组8只,一次性分别照射X射线(双眼同时照射)5、15、25 Gy.实验对照组24只,分成3组每组8只,分别作为5、15、25 Gy的对照组行假照射(照射光线中不含X射线,其它条件分别与对应各照射组相同)。每只大鼠随机取一只眼睛(本实验取右眼)为研究对象。所有大鼠分别于照射后1、3、5、7、15、30、45、60、90 d,腹腔注射3.6%水合氯醛麻醉后,用双星明滴眼液充分散瞳,裂隙灯显微镜观察并记录晶状体混浊情况;于照射后3个月将大鼠处死,取晶状体作匀浆,取其上清液(可溶性晶体总蛋白)采用免疫印迹法(Western blot)检测可溶性αB-晶体蛋白的含量。 结果:观察3个月,正常对照组、各实验对照组和5 Gy照射组大鼠的晶状体均未出现混浊。15 Gy照射组于照射后45 d晶状体后囊和后皮质开始出现细小、散在白点状混浊,继而融合成不均匀较密集点状、片状混浊,进一步发展后皮质出现较均匀致密混浊,前皮质、前囊和核部轻度混浊。到3个月时62.5%的晶状体达Ⅱ期混浊,37.5%达Ⅲ期混浊。25 Gy照射组亦出现类似变化,但是晶体发生混浊的时间更早、速度更快、混浊更明显。于照射后30 d后囊即开始出现点状混浊,此后病变迅速发展,到45 d时即有37.5%的晶体全部混浊。到3个月的时候除1例为后囊下明显混浊外,其余7例晶状体均完全混浊;15、25 Gy照射组可溶性晶体总蛋白浓度低于其它各组(P0.05);实验对照组之间可溶性αB-晶体蛋白含量的差异无统计学意义(P0.05),各实验照射组可溶性αB-晶体蛋白含量分别较其实验对照组降低,差异有统计学意义(P0.05) ,3个实验照射组可溶性αB-晶体蛋白含量随照射剂量的增加而逐渐降低,其差异有显著统计学意义,(P0.05). 结论:大剂量X射线在短期内即可引起晶状体混浊;放射性白内障的形成速度和程度与射线剂量成正相关,在放射性白内障中晶状体内可溶性αB-晶体蛋白表达减少,在放射性白内障形成中起重要作用。
[Abstract]:Objective: to establish an animal model of radioactive cataract in the eyes of S-D rats by X-ray irradiation, and to observe the relationship between the dose of X-ray and the severity of cataract. The expression of soluble 伪 B crystal protein in rat lens irradiated with different doses of X ray was detected and the relationship between 伪 B crystal protein and radiation cataract formation was investigated. Methods: eight rats were selected as the normal control group before irradiation. 24 rats in the experimental group were divided into 3 groups with 8 rats in each group. 25 Gy.The experimental control group (n = 24) was divided into 3 groups (n = 8 in each group). The other conditions were the same as the corresponding irradiation groups. One eye was randomly selected from each rat (right eye in this experiment). 3.6% chloral hydrate was injected intraperitoneally for 90 days and the lens opacity was observed and recorded by slit lamp microscope. The rats were killed 3 months after irradiation and the lens was taken as homogenate. The supernatant (soluble crystal total protein) was used to detect the content of soluble 伪 B crystal protein by Western blot. Results: the control group was observed for 3 months. The lens of the experimental control group and the 5 Gy irradiation group did not appear opacity. The lens posterior capsule and posterior cortex of the 15 Gy irradiation group began to appear fine and scattered in the white dot shape after 45 days of irradiation. Then fusion into heterogeneous more dense dot shape, flake turbidity, after further development of the cortex appeared more homogeneous dense turbidity, precortex. The anterior capsule and nucleus were slightly opacified. By 3 months, 37.5% of the 62.5% lenses reached stage 鈪，

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