Decorin对人僵直膝关节滑膜囊成纤维样滑膜细胞增殖及蛋白合成的抑制作用

发布时间：2018-01-29 18:04

  本文关键词： 膝关节僵直 成纤维细胞样滑膜细胞 核心蛋白多糖 I型胶原蛋白　出处：《中国矫形外科杂志》2015年13期 　论文类型：期刊论文

【摘要】：[目的]探讨重组核心蛋白多糖(decorin)对培养的人僵直膝关节滑膜成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)的增殖和Ⅰ型胶原蛋白及mRNA合成的影响,为Decorin治疗人膝关节僵直提供理论依据。[方法]分离培养人僵直膝关节滑膜FLS,分别加入不同质量浓度(10μg/ml,0.1μg/ml)的Decorin;培养24、48、72h后用二甲基噻唑二苯基四唑溴盐(MTT)法测定FLS的增殖速度;用流式细胞仪检测FLS的周期和凋亡;用RTPCR法检测FLS合成Ⅰ型前胶原蛋白(PcⅠ)mRNA的含量,westem Blot检测Ⅰ型胶原蛋白的合成量,并与对照组比较。[结果]10μg/ml的Decorin可有效抑制滑膜FLS的增殖(P0.05),明显增加FLS处于G0/G1期的百分比(P0.05),并通过下调PcⅠmRNA的表达,在转录水平上抑制PcⅠ的合成;实验组和对照组均未检测到终末期凋亡细胞。[结论]Decorin可抑制人僵直膝关节滑膜FLS的增殖及PcⅠmRNA的表达和Ⅰ型胶原蛋白合成,在防治膝关节僵直中起一定的作用。
[Abstract]:[Objective] to investigate the effect of recombinant core proteoglycan decorin (RCP) on cultured human rigid synovial fibroid synovial cells. Fibroblast-like synoviocytes. The effects of FLS on the synthesis of collagen type I and mRNA may provide theoretical basis for the treatment of knee joint stiffness with Decorin. [Methods] Human knee joint synovium FLS was isolated and cultured, and 10 渭 g / ml of 10 渭 g / ml (0.1 渭 g / ml) of Decorin was added respectively. The proliferation rate of FLS was determined by dimethyl thiazole diphenyl tetrazolium bromide salt method after 72 hours culture. The cycle and apoptosis of FLS were detected by flow cytometry. The content of procollagen 鈪，

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