不开腹单纯膀胱内注射制备急性肾盂肾炎大鼠模型

发布时间：2018-01-31 21:31

本文关键词： 急性肾盂肾炎大肠杆菌模型大鼠　出处：《安徽医药》2015年05期 　论文类型：期刊论文

【摘要】：目的研究不开腹状态下经尿道直接膀胱内注射大肠杆菌制备急性肾盂肾炎大鼠模型。方法 24只雌性SD大鼠随机分为对照组、感染3 d组及感染7 d组。大鼠禁水24 h,10%水合氯醛麻醉后,经尿道注入108cfu·m L-1活性大肠杆菌(E.coli)约0.5 m L,同时细线结扎尿道口2 h后松开。测量大鼠处死前体重及双侧肾重,计算肾重指数。膀胱内尿液及肾脏匀浆行细菌培养,肾脏HE染色行病理学检查。结果感染各组大鼠肾脏均较对照组增大,肾重系数升高,具有统计学意义,而感染3d组与7 d组间无明显差异。感染3 d组及7 d组大鼠膀胱尿培养阳性率分别为100%和62.5%,肾脏细菌培养阳性率分别为100%和75%,与对照组相比,P0.05,差异具有统计学意义。光镜可见感染3 d组肾盂黏膜下及间质区有大量中性粒细胞及淋巴细胞浸润,毛细血管扩张;感染7 d组可见肾盂黏膜下大量淋巴细胞浸润及少量嗜酸性粒细胞。两组肾小球及肾小管结构均未见明显变化。结论不开腹状态下经尿道直接膀胱内注射大肠杆菌可成功制备急性肾盂肾炎大鼠模型。
[Abstract]:Objective to study the rat model of acute pyelonephritis induced by direct intravesical injection of Escherichia coli through urethra. Methods 24 female SD rats were randomly divided into control group. Rats were anesthetized with 10% chloral hydrate for 24 h after exposure to water for 3 days and 7 days. About 0.5 mL E. coli was injected into the urethra with 108 CFU 路mL -1 active Escherichia coli. At the same time, the urethral orifice was ligated for 2 hours. The body weight and bilateral renal weight of the rats were measured before death, and the renal weight index was calculated. The urine and kidney homogenate of the bladder were cultured. Results compared with the control group, the kidney of the infected rats increased and the renal weight coefficient increased, which was statistically significant. However, there was no significant difference between the 3 d and 7 d groups. The positive rates of bladder urine culture were 100% and 62.5% in the 3 d and 7 d groups, respectively. The positive rates of renal bacterial culture were 100% and 75 respectively, compared with the control group (P0.05). The difference was statistically significant. Light microscopy showed a large number of neutrophil and lymphocyte infiltration and capillary dilatation in the submucous and interstitial areas of renal pelvis in the 3 d infection group. Infection 7. In group d, a large number of lymphocytes and a small amount of eosinophils were observed in the submucosa of renal pelvis. There were no significant changes in glomerular and tubular structures in both groups. Conclusion the direct intravesical injection of Escherichia coli through urethra can be formed without laparotomy. The rat model of acute pyelonephritis was established.
【作者单位】：安徽医科大学解放军306临床学院;中国人民解放军306医院肾内科;北京市顺义区中医院肾内科;
【基金】：全军“十二五”重点课题(No AWS11J013;No BWS11J051)
【分类号】：R692.7;R-332
【正文快照】： 急性肾盂肾炎最常见的感染细菌为大肠杆菌。各种原因引起的膀胱输尿管反流和肾内反流是致病菌进一步上行引起肾脏炎症及肾盂肾炎的重要病理基础。基于此原理,目前多采用大鼠单侧输尿管“半结扎”,膀胱内注射大肠杆菌同时尿道口夹闭的方法制作急性肾盂肾炎的大鼠模型[1]。该方

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