乙醇、乙醛对动物依赖行为及心肌电生理特性影响的研究

发布时间：2018-02-07 15:09

本文关键词： 乙醇乙醛心肌细胞电生理学酒依赖　出处：《湖南师范大学》2008年硕士论文　论文类型：学位论文

【摘要】： 人类有着较长时间的酿酒饮酒历史,但长期、大量摄入酒精的行为也给酗酒者带来了一系列的安全和健康问题。关于饮酒对人或动物行为、生理特性的影响,以往的研究多集中于关注乙醇的作用,但乙醇体内代谢的中间产物乙醛在酒依赖中的作用研究依不太清楚。本研究从心肌细胞电生理学和动物依赖行为两个方面比较乙醇、乙醛的作用差异。我们测定了急性乙醇、乙醛浸润对蟾蜍心肌细胞电生理特性的影响,结果表明,乙醇可缩短心室肌细胞动作电位的时程(APD_(90)),提高静息电位(RP);对动作电位峰值(APA),动作电位0期去极化速度(Vmax)影响不显著。而在乙醛作用下,APD_(90)延长,APA、Vmax降低,低浓度乙醛影响下RP的绝对值增大,而当浓度增后其绝对值减小。通过条件位置偏好(CPP)和条件味觉偏好(CTP)实验研究小鼠较长时间摄取乙醇、乙醛后其偏好性行为的改变。经乙醇预处理,训练8次后,小鼠表现出对乙醇的条件位置偏好,而经乙醛预处理和训练的小鼠则对乙醛无明显条件偏好行为;当用乙醇、乙醛混合训练乙醇依赖性小鼠时,其位置偏好行为减弱;乙醇预处理的小鼠味觉偏好乙醇,而当乙醇中加入少量乙醛时,其味觉偏好现象则显著减弱。乙醛训练后的小鼠不表现对乙醇的味觉偏好,但选择摄入乙醛或乙醇、乙醛混合溶液的量有所增加。乙醇、乙醛对离体心肌标本电生理特性的急性影响和动物行为的影响有一定的相似性,又各有其特点。乙醛的急性影响效应浓度较乙醇低,在小鼠酒依赖行为中,乙醛也可能存在一定促进作用。
[Abstract]:People have a long history of wine drinking, but for a long time, the behavior of heavy alcohol intake also brings a series of safety and health problems to alcoholics. Previous studies have focused on the role of ethanol, but the role of acetaldehyde, the intermediate product of ethanol metabolism, in alcohol dependence is not clear. This study compares ethanol from the aspects of cardiomyocyte electrophysiology and animal dependent behavior. The effect of acetaldehyde is different. The effects of acute ethanol and acetaldehyde infiltration on the electrophysiological characteristics of toad cardiomyocytes were determined. Ethanol can shorten the duration of action potential of ventricular myocytes and increase the resting potential of APD90, but it has no significant effect on the peak value of action potential and the depolarization velocity of action potential in phase 0. However, under the action of acetaldehyde, APDV _ (90) prolongs the decrease of APAV _ (max). The absolute value of RP increases under the influence of low concentration of acetaldehyde, but decreases when the concentration of acetaldehyde increases. The effects of ethanol intake and acetaldehyde on the preference behavior of mice were studied by means of CPP (conditional position preference) and CTP (conditioned taste preference). After ethanol pretreatment and training for 8 times, the mice showed a conditional position preference for ethanol. However, the mice pretreated and trained by acetaldehyde had no conditioned preference for acetaldehyde; when ethanol and acetaldehyde were mixed to train ethanol-dependent mice, their position preference was weakened, and the taste preference of ethanol pretreated mice was decreased. However, when a small amount of acetaldehyde was added into ethanol, the taste preference was significantly decreased. The mice trained with acetaldehyde did not show taste preference for ethanol, but the amount of acetaldehyde mixed solution increased when they chose to eat acetaldehyde or ethanol. The acute effects of ethanol and acetaldehyde on the electrophysiological characteristics and animal behavior of isolated myocardial specimens were similar, and each had its own characteristics. The acute effect concentration of acetaldehyde was lower than that of ethanol, and it was found in the alcoholic behavior of mice. Acetaldehyde may also have some promotive effect.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R363

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