电离辐射对小鼠免疫器官调节性T细胞及其相关分子表达的影响

发布时间：2018-02-07 16:45

本文关键词： 电离辐射调节性T细胞 Foxp3 TGF-β1 免疫反应　出处：《吉林大学》2009年硕士论文　论文类型：学位论文

【摘要】： 本文初步研究了调节性T细胞(Treg细胞)及其相关分子Foxp3和TGF-β1在不同时间和不同剂量电离辐射作用下,从基因水平、蛋白水平和细胞水平探讨其表达的时间效应和剂量效应变化。实验结果显示,在胸腺细胞中CD4+CD25+细胞数不管在低剂量还是高剂量辐射作用下均增多,但脾细胞中的CD4+CD25+细胞数只在高剂量辐射作用下明显增多;这很可能和脾细胞中含有的T淋巴细胞以成熟T细胞为主有关。 Foxp3是Treg细胞的特异性标志,对Treg细胞的分化有着重要作用,尤其是对Treg细胞功能的发挥是必不可少的;本实验提示,在低剂量照射条件下其表达没有上调,甚至略有下降,这也说明了在低剂量照射时Treg细胞的免疫负性调节功能没有激活或上调。高剂量照射条件下Foxp3表达明显上调,提示Treg细胞被激活,行使免疫负性调节。因此,不同剂量电离辐射对机体的免疫抑制反应的关键可能在Foxp3分子的变化上。 TGF-β1在CD4+CD25+Treg细胞的发育分化成熟中起到了决定性的作用,在本实验中TGF-β1与CD4+CD25+Treg细胞不论是在时间效应还是在剂量效应变化中,都有类似的变化,这在一定程度上解释了Treg细胞在辐射条件下表达变化的趋势,这印证了TGF-β1对CD4+CD25+Treg细胞的发育成熟所起到的作用;大剂量辐射作用下CD4+CD25+TGF-β1促进Treg细胞的数量增加,为其抑制功能增强提供了条件。
[Abstract]:In this paper, we preliminarily studied regulatory T cell / Treg cell) and its related molecules Foxp3 and TGF- 尾 _ 1 at different time and different doses of ionizing radiation. The effects of time and dose on the expression of CD4 CD25 in thymocytes were investigated at the protein and cell levels. The results showed that the number of CD4 CD25 cells in thymocytes was increased in both low and high doses of radiation. However, the number of CD4 CD25 cells in spleen cells increased only under high dose radiation, which may be related to the majority of T lymphocytes contained in spleen cells. Foxp3 is a specific marker of Treg cells and plays an important role in the differentiation of Treg cells, especially the function of Treg cells. This also indicates that the immuno-negative regulatory function of Treg cells is not activated or upregulated under low dose irradiation. The expression of Foxp3 is obviously up-regulated under high dose irradiation, suggesting that Treg cells are activated and exercise immuno-negative regulation. The key to the immunosuppressive response of different doses of ionizing radiation may lie in the changes of Foxp3 molecules. TGF- 尾 1 plays a decisive role in the development, differentiation and maturation of CD4 CD25 Treg cells. In this experiment, TGF- 尾 1 and CD4 CD25 Treg cells have similar changes in both time and dose effects. This may explain to some extent the tendency of expression change of Treg cells under radiation, which confirms the effect of TGF- 尾 1 on the development and maturation of CD4 CD25 Treg cells, and CD4 CD25 TGF- 尾 1 promotes the increase of Treg cells under high dose radiation. It provides the condition for the enhancement of its inhibitory function.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392

【参考文献】