饮食限制对雌性老龄大鼠抗衰老效应的实验研究

发布时间：2018-02-09 12:47

本文关键词： 饮食限制细胞自噬生存分析氧化应激抗衰老　出处：《山西医科大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的：观察不同饮食方式下雌性老龄大鼠发生细胞自噬的差异,同时研究饮食限制对雌性老龄大鼠血清超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde, MDA)含量的影响,分析实验动物的自然寿命及其生存曲线,探讨饮食限制抗衰老的生物学效应及机制。方法：45只24月龄雌性健康SD大鼠随机、平均分成三组：对照组(正常饮食组)、隔日禁食组(一日禁食一日进食组)和两日禁食组(持续禁食两日,持续进食五日组)。不同饮食方式干预三月后采集标本。透射电镜观察肝脏细胞自噬泡并对细胞的自噬活性进行定量分析；试剂盒测定大鼠血清SOD活性和MDA含量；绘制生存曲线,进行生存分析；对细胞自噬活性和大鼠生存时间进行关联性分析；对血清SOD活性、MDA含量和大鼠生存时间进行关联性分析。结果：与对照组比较,隔日禁食组和两日禁食组大鼠肝脏细胞自噬泡占胞浆总面积的比值增高,差异均有统计学意义(P0.01),而此两组间差别无统计学意义(P0.05)。与对照组比较,隔日禁食组SOD活性增高(P0.01),MDA含量降低(P0.01)；两日禁食组SOD活性增高(P0.05),MDA含量降低(P0.01),而此两组间SOD活性及MDA含量差别均无统计学意义(P0.05)。生存分析结果显示三组生存曲线不同,与对照组比较,隔日禁食组和两日禁食组生存率增高,差异均有统计学意义(P0.01),而此两组间差别无统计学意义(P0.05)。对细胞自噬活性和大鼠生存时间进行关联性分析,结果为rS=0.903,表明细胞自噬活性和大鼠生存时间之间呈线性正相关。对血清SOD活性和大鼠生存时间进行关联性分析,结果为rS=0.717,同时对MDA含量和大鼠生存时间进行关联性分析,结果为rS=—0.745,表明SOD活性和大鼠生存时间之间呈线性正相关,而MDA含量和大鼠生存时间之间呈线性负相关。结论：1细胞自噬呈高活性状态有利于大鼠寿命的延长；饮食限制导致的寿命延长与细胞自噬相关。2对老年大鼠进行饮食限制,能明显提高SOD活性,降低MDA含量,改善氧化应激水平。3饮食限制通过诱导细胞自噬、改善氧化应激等生物学机制发挥其抗衰老效应。
[Abstract]:Objective: to observe the difference of autophagy in aged female rats under different dietary patterns, and to study the effects of dietary restriction on the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDAs) in serum of aged female rats. The natural life span and survival curve of experimental animals were analyzed, and the biological effects and mechanism of dietary restriction on anti-aging were discussed. Methods 45 healthy female SD rats aged 24 months were randomly divided into three groups: control group (normal diet group), fasting group (1st fasting 1st group) and two day fasting group (two days fasting for two days), the control group (normal diet group), the second day fasting group (1st fasting 1st group) and the two day fasting group (two days). The liver cells were observed by transmission electron microscope (TEM) and the autophagy activity was quantitatively analyzed. The serum SOD activity and MDA content were measured by kit, and the survival curve was drawn. The relationship between autophagy activity and survival time of rats was analyzed, and the relationship between serum SOD activity and survival time of rats was analyzed. Results: compared with the control group, the ratio of hepatic autophagy to total cytosolic area in the two groups was significantly higher than that in the control group, but there was no significant difference between the two groups. The activity of SOD was increased in the fasting group on the next day and the content of SOD was decreased in the fasting group on the following day. The activity of SOD in the two groups was increased and the content of MDA was decreased in the two groups. There was no significant difference in the activity of SOD and the content of MDA between the two groups. The survival analysis showed that the survival curves of the three groups were different, the results of survival analysis showed that the survival curves of the three groups were different. Compared with the control group, the survival rate of the two groups was significantly higher than that of the control group (P 0.01), but there was no significant difference between the two groups. The relationship between the cell autophagy activity and the survival time of rats was analyzed. The results showed that there was a linear positive correlation between autophagy activity and survival time of rats. The correlation between serum SOD activity and survival time of rats was analyzed. The results were as follows: rS0. 717. The correlation between the content of MDA and the survival time of rats was also analyzed. The results showed that there was a positive linear correlation between the activity of SOD and the survival time of rats, but a negative correlation between the content of MDA and the survival time of rats. Conclusion the hyperactive state of autophagy of 1 / 1 cell is beneficial to the prolongation of the life span of rats, and the prolongation of life span caused by dietary restriction and the dietary restriction on the aged rats induced by dietary restriction can obviously increase the activity of SOD and decrease the content of MDA. Improving oxidative stress level 3. 3 dietary restriction exerts its anti-aging effect by inducing autophagy and improving oxidative stress and other biological mechanisms.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R339.38

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