肾囊阻滞对胃肠运动双向调节的实验研究

发布时间：2018-02-09 15:05

本文关键词： 肾囊阻滞胃内残留率小肠推进率胃动素一氧化氮　出处：《中南大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的：以胃肠动力异常的大鼠为研究对象,观察肾囊阻滞对胃内残留率、小肠推进率、脑肠肽激素以及肠神经递质的变化。探讨肾囊阻滞调节胃肠动力的规律及机制。方法： 1、将72只雄性SD大鼠随机均分为3大组,组A为非模型组,组B为腹腔注射阿托品制作的大鼠胃肠运动抑制模型,组C为腹腔注射新斯的明制作的大鼠胃肠运动亢进模型。每大组又随机均分为3小组,每小组8只大鼠,组A1、B1、C1为假手术组,即仅暴露肾脏及肾囊,组A2、B2、C2为肾囊注射生理盐水组,组A3、B3、C3为肾囊注射利多卡因组。 2、各组大鼠经肾囊阻滞后,予以半固体食物灌胃。通过称胃全重及胃净重计算胃内残留率,并用炭末法测量小肠推进率,观察各组大鼠胃内残留率、小肠推进率的变化。 3、用放射免疫法检测胃窦、下丘脑组织中脑肠肽胃动素(MTL)的含量,观察各组大鼠MTL的变化。 4、用硝酸还原酶法检测结肠组织中神经递质一氧化氮(NO)的含量,观察各组大鼠NO的变化。结果： 1、与组A1相比,组Bl的胃内残留率显著升高,小肠推进率、胃窦和下丘脑中MTL含量显著降低(p0.05),组C1的胃内残留率显著降低,小肠推进率、胃窦和下丘脑中MTL含量显著升高(p0.05)。 2、组A1与组A2、组B1与组B2以及组C1与组C2相比,胃内残留率、小肠推进率以及胃窦和下丘脑中MTL含量无显著性差异(p0.05)。 3、在非模型组中,组A1与组A3相比,胃窦MLT含量无显著性差异(p0.05),组A3的胃内残留率显著降低,小肠推进率、下丘脑MTL的含量显著增高(p0.05),组A2与组A3相比,胃内残留率、胃窦和下丘脑MLT含量无显著差异(p0.05),组A3的小肠推进率显著升高(p0.05)；在抑制模型组中,与组B1和组B2比较,组B3的胃内残留率显著降低,小肠推进率、胃窦和下丘脑MTL含量显著增高(p0.05)；在亢进模型组中,与组C1和组C2比较,组C3的胃内残留率显著增高,小肠推进率、胃窦和下丘脑MTL含量显著降低(p0.05)。 4、各组大鼠结肠NO含量无显著性差异(p0.05)。结论： 1、肾囊阻滞可能通过升高胃肠动力低下动物模型体内MTL含量,加快阿托品抑制模型的胃排空、小肠推进,也可降低胃肠动力亢进动物模型体内MTL含量,减慢新斯的明亢进模型的胃排空、小肠推进,提示肾囊阻滞可通过神经体液调节对胃肠运动起到双向调节作用。 2、肾囊阻滞对胃肠道正常状态氮能神经NO含量无明显影响。
[Abstract]:Aim: to observe the changes of residual rate in stomach, small intestine propulsion rate, brain intestinal peptide hormone and intestinal neurotransmitter in rats with abnormal gastrointestinal motility, and to investigate the regulation and mechanism of renal capsule block on gastrointestinal motility. Methods:. 1. 72 male Sprague-Dawley rats were randomly divided into 3 groups. Group A was a non-model group and group B was a gastrointestinal motility inhibition model made by intraperitoneal injection of atropine. Group C was induced by intraperitoneal injection of neostigmine. Each group was randomly divided into 3 groups (8 rats in each group). Group A _ (1) B _ (1) C _ (1) was sham-operated group, that is, only kidney and renal sac were exposed, group A _ (2) B _ (2) C _ (2) was injected normal saline into renal capsule, and group A _ (2) B _ (2) C _ (2) was injected with normal saline. Group A _ 3 B _ 3 C _ 3 was injected with lidocaine into renal capsule. 2. Rats in each group were fed with semi-solid food after renal capsule block. The gastric residual rate was calculated by weighing the whole stomach weight and the net gastric weight, and the small intestinal propulsion rate was measured by carbon powder method. The changes of gastric residual rate and small intestinal propulsion rate were observed in each group. 3. The contents of motilin motilin in antrum and hypothalamus were detected by radioimmunoassay, and the changes of MTL were observed in each group. 4. Nitrate reductase method was used to detect the content of nitric oxide (no) in colon tissue, and the changes of no in each group were observed. Results:. 1. Compared with group A1, the gastric residual rate of group B1 was significantly increased, the intestinal propulsion rate, the MTL content in gastric antrum and hypothalamus was significantly decreased, the gastric residual rate in group C1 was significantly decreased, the intestinal propulsive rate and MTL content in antrum and hypothalamus were significantly increased (P 0.05). 2There was no significant difference between group A1 and group A2, group B1 with group B2 and group C1 with group C2 in gastric residual rate, intestinal propulsion rate and MTL content in antrum and hypothalamus (p0.05). 3. In the non-model group, there was no significant difference in gastric antral MLT content between group A1 and group A3 (p0.05). The gastric residual rate of group A3 was significantly lower, the rate of small intestinal propulsion and the content of MTL in hypothalamus was significantly higher than that of group A _ 2 and group A _ 3, and the intragastric residual rate of group A _ 2 was significantly higher than that of group A _ 3. There was no significant difference in MLT content between antrum and hypothalamus (p0.05), but the small intestinal propulsive rate of group A _ 3 was significantly higher than that of group B _ 1 and B _ 2.Compared with group B _ 1 and group B _ 2, the gastric residual rate of group B _ 3 was significantly lower than that of group B _ 2, and the rate of small intestinal propulsion was significantly lower in group A _ 3. The contents of MTL in antrum and hypothalamus were significantly increased in the hyperactive model group compared with group C1 and group C2, the gastric residual rate of group C _ 3 was significantly higher, the rate of small intestinal propulsion and the content of MTL in antrum and hypothalamus were significantly lower than those in group C _ 1 and group C _ 2, and the contents of MTL in antrum and hypothalamus were significantly lower than those in group C _ 2. 4, there was no significant difference in no content in colon of rats in each group (P 0.05). Conclusion:. 1. Renal sac block may increase the MTL content in the gastrointestinal hypokinetic animal model, accelerate the gastric emptying and intestinal propulsion of the atropine inhibition model, and decrease the MTL content in the hypergastrointestinal hyperkinetic animal model. The decrease of gastric emptying and small intestinal propulsion in neostigmine model suggests that renal capsule block can regulate gastrointestinal motility through neurohumoral regulation. 2. Renal capsule block had no effect on no content in normal gastrointestinal tract.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R333

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