贵州省结核分枝杆菌临床分离株的基因分型及耐药性分析

发布时间：2018-02-10 11:20

本文关键词： 结核分枝杆菌 RD 基因分型耐药性　出处：《中国病原生物学杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的了解贵州省结核分枝杆菌临床分离株的基因分型及耐药情况。方法收集2014年贵州省结核分枝杆菌临床分离株260株,采用比例法进行药敏试验;以16SrRNA基因和MTP40基因为对照,通过PCR检测RD105基因进行北京型鉴定;采用NTF分析法对北京家族菌株进行古典型和现代型分型。结果 260株结核分枝杆菌临床分离株中北京家族菌株259株(99.6%),非北京家族菌株1株(0.4%),其中北京家族古代型占96.9%(251/259),现代型占3.1%(8/259)。北京家族菌株古代型对异烟肼、利福平、链霉素和乙胺丁醇的耐药率分别为33.9%、30.3%、31.8%和21.1%,耐多药率为29.5%;北京家族菌株现代型未检出耐多药菌株,与北京家族菌株古代型的耐多药率比较,差异有统计学意义(χ~2=14.07,P0.05)。结论贵州省结核分枝杆菌主要流行株为北京家族古代型,北京家族古代型耐多药率高于现代型。
[Abstract]:Objective to investigate the genotyping and drug resistance of clinical isolates of Mycobacterium tuberculosis in Guizhou Province. Methods in 2014, 260 clinical isolates of Mycobacterium tuberculosis in Guizhou Province were collected and tested for drug sensitivity by proportional method, 16s rRNA gene and MTP40 gene were used as controls. The RD105 gene was identified by PCR. Results among 260 clinical isolates of Mycobacterium tuberculosis, 259 strains were isolated from Beijing family and 1 strain was non-Beijing family strain. 96. 9 / 251 / 259, 3. 1 / 259. Ancient strains of the Beijing family were of isoniazid. The resistance rates of rifampicin, streptomycin and ethambutanol were 33.9% and 21.1%, respectively, and the multidrug resistance rate was 29.5%. Conclusion the main prevalent strains of Mycobacterium tuberculosis in Guizhou province are the ancient strains of Beijing family, and the rate of multidrug resistance in ancient Beijing type is higher than that in modern type.
【作者单位】：贵州医科大学微生物学教研室;贵阳市肺科医院;
【基金】：贵州省社会发展攻关项目[黔科合SY(2013)3060]
【分类号】：R446.5;R378.911

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