分泌表达结核分枝杆菌Ag85B蛋白的重组卡介苗的构建和免疫学特性的初步研究

发布时间：2018-03-03 18:20

本文选题：结核杆菌　切入点：重组卡介苗　出处：《吉林大学》2008年硕士论文　论文类型：学位论文

【摘要】： 结核病是由结核分枝杆菌引起的以呼吸道传播为主的慢性传染病,结核病被WHO称为唯一的一种全球性紧迫性疾病。据WHO报道,全世界目前约有1?3人口潜伏感染结核杆菌,2005年新增病例约为880万,死亡160万。贫穷、人口增加、流动人口增多、耐药菌株及艾滋病流行等使结核病雪上加霜。我国结核患者数量居世界第二位,我国现有结核感染者4亿人。卡介苗是目前世界上唯一可以应用的预防结核病的疫苗。但卡介苗在不同地区不同人群中存在着差异。卡介苗可以有效预防儿童型结核感染,但不能预防成人型肺结核,其预防作用从0-80%不等。由于BCG预防结核病的不足,以及当前TB流行的严重性,因此研究新的疫苗用于结核的预防已成为当前国内外研究的热点。本研究主要构建了表达Ag85B的重组卡介苗,以弥补传统卡介苗在预防结核病的不足。用分泌表达Ag85B蛋白的rBCG和普通BCG分别经皮下免疫BALB/c小鼠,评价各组免疫小鼠的特异性体液免疫和细胞免疫水平。实验结果表明分泌表达Ag85B蛋白的rBCG与普通BCG相比可以大量分泌抗原Ag85B;长时间免疫后的检测结果表明rBCG比BCG刺激较强的体液免疫应答,但在细胞免疫反应上不是很强。因此,rBCG的免疫保护作用还有待于进一步研究。
[Abstract]:Tuberculosis is a chronic infectious disease mainly transmitted by respiratory tract caused by Mycobacterium tuberculosis. Tuberculosis is regarded as the only global urgent disease by WHO. According to WHO, there are about 1? In 2005, the number of new cases was about 8.8 million and the death rate was 1.6 million. Poverty, population increase, floating population increasing, drug-resistant strains and the prevalence of HIV / AIDS made TB worse. The number of TB patients in China ranked second in the world. There are 400 million people infected with tuberculosis in China. BCG vaccine is the only vaccine available in the world to prevent tuberculosis. But BCG vaccine varies from region to region. BCG vaccine can effectively prevent tuberculosis infection in children, but it can not prevent tuberculosis in adults. The preventive effects range from 0 to 80%. Because of the insufficient prevention of TB by BCG and the severity of the current TB epidemic, Therefore, the research on the application of new vaccines to the prevention of tuberculosis has become a hot topic at home and abroad. In this study, recombinant BCG vaccine expressing Ag85B was constructed. In order to make up for the deficiency of traditional BCG vaccine in preventing tuberculosis, BALB/c mice were immunized subcutaneously with rBCG and BCG which secreted Ag85B protein to evaluate the specific humoral immunity and cellular immunity of each group of immunized mice. The results showed that rBCG secreting Ag85B protein could secrete Ag85B in a large amount compared with ordinary BCG, and the results of long time immunization showed that rBCG stimulated a stronger humoral immune response than BCG. But the cellular immune response is not very strong, so the protective effect of rBCG remains to be further studied.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392.11

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