七氟醚、异氟醚预处理对大鼠肝脏缺血再灌注损伤及能量代谢的影响

发布时间：2018-03-03 21:07

本文选题：七氟醚　切入点：异氟醚　出处：《广西医科大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的观察七氟醚、异氟醚预处理对大鼠肝脏缺血再灌注损伤以及能量代谢的影响。方法选择SD雄性大鼠60只,体重240～250g,随机分为三组,肝脏缺血再灌注组(IR),异氟醚预处理组(Iso),七氟醚预处理组(Sev),每组20只。IR组:制作可靠的肝缺血模型,肝脏缺血30min,于肝脏缺血前(T_1)、肝脏缺血后再灌注30min(T_2)、120min(T_3)测各组大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)乳酸脱氢酶(LDH)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的含量以及T_3时点肝脏组织中谷胱甘肽还原酶(GR)、丙二醛(MDA)的含量、超氧化物歧化酶(SOD)活性以及肝脏的ATP含量,留取部分肝脏做HE病理染色。Sev组:与相应麻醉气体挥发罐相连接的密闭容器,监测密闭容器的七氟醚浓度,当七氟醚浓度稳定于2%,将大鼠置入密闭容器内保持自主呼吸30min,然后取出大鼠晾于空气中10min,余步骤同IR组;Iso组:与相应麻醉气体挥发罐相连接的密闭容器,监测密闭容器的异氟醚浓度,当异氟醚浓度稳定于1.5%,将大鼠置入密闭容器内保持自主呼吸30min,然后取出大鼠晾于空气中10min,余步骤同IR组。结果IR组、Sev组、Iso组分别与T_1时点相比较T_2、T_3时点血清ALT、AST、LDH明显升高,差异有显著性(P0.05);Sev组和Iso组分别与IR组相比较在T_1时点血清ALT、AST、LDH差异无显著性(P0.05),Sev组和Iso组分别与IR组相比较在T_2、T_3时点血清ALT、AST、LDH明显降低,差异有显著性(P0.05);IR组、Sev组、Iso组分别与T_1时点相比较T_2、T_3时点血清TNF-α、IL-1β明显升高,差异有显著性(P0.05);Sev组与IR组相比较在T_2、T_3时点血清白细胞介素-1β明显降低,差异有统计学意义(P0.05),Iso组与IR组相比较在T_2、T_3时点血清IL-1β差异无统计学意义(P0.05);Sev组和Iso组分别与IR组相比较在T_2、T_3时点血清TNF-α差异无统计学意义(P0.05);Sev组、Iso组分别与IR组相比较在T_3时点肝组织MDA明显降低,差异有统计学意义(P0.05),Sev组、Iso组分别与IR组相比较在T_3时点肝组织SOD、GR明显升高,差异有统计学意义(P0.05);Sev组、Iso组分别与IR组相比较在T_3时点肝脏组织ATP含量明显升高,差异有统计学意义(P0.05);肝脏病理与正常肝组织相比较,发生缺血相应改变,Sev组、Iso组、IR组间差异不明显。结论七氟醚和异氟醚预处理能减轻大鼠肝脏缺血再灌注损伤,能改善缺血再灌注损伤肝脏组织的能量代谢。
[Abstract]:Aim to observe the effects of sevoflurane and isoflurane preconditioning on hepatic ischemia-reperfusion injury and energy metabolism in rats. Methods Sixty SD male rats weighing 240 ~ 250g were randomly divided into three groups: hepatic ischemia reperfusion group, isoflurane preconditioning group, sevoflurane preconditioning group and sevoflurane preconditioning group. 30 min after hepatic ischemia, 30 min after hepatic ischemia and 30 min after liver reperfusion, the levels of serum alanine aminotransferase (alt), aspartate aminotransferase (AST) lactate dehydrogenase (LDH), tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾) and liver tissue in T3 were measured. The content of glutathione reductase GRN, malondialdehyde (MDA), The activity of superoxide dismutase (SOD) and the content of ATP in liver were collected for HE pathological staining. Group Sev: sealed container connected with the volatile tank of the corresponding anesthetic gas to monitor the concentration of sevoflurane in the sealed container. When the concentration of sevoflurane was stable at 2%, the rats were placed in an airtight container for 30 minutes and then dried in the air for 10 minutes. The remaining steps were the same as IR group and Iso group: an airtight container connected with the corresponding anesthetic gas volatile tank. The concentration of isoflurane in the closed vessel was monitored. When the concentration of isoflurane was stable at 1.5, the rats were placed in the closed container for 30 minutes, then the rats were taken out to air for 10 minutes. The remaining steps were the same as that in the IR group. Results Serum alt ASTT LDH was significantly increased at T _ 2 / T _ T _ 3 in IR group, Sev group and Iso group, compared with T _ 1 time point, respectively. There was no significant difference in serum alt ASTT LDH between Iso group and Iso group, compared with IR group. There was no significant difference in serum alt ASTT LDH between P0 05 sev group and Iso group compared with IR group at T0. 05 and T 0. 05%, respectively, compared with IR group, the serum alt AST LDH was significantly decreased at T 2 / T 3 time point in T _ 2 / T _ 2 / T _ 3 group, compared with that in T _ 2 / T _ 2 / T _ 3 group, and no significant difference was found between the two groups. There was a significant difference in serum TNF- 伪 and IL-1 尾 between the two groups (P 0.05 / IR) and Sev / Iso (P < 0.05), compared with that at T _ (1) and T _ (2) / T _ (3), respectively. The serum levels of interleukin-1 尾 (IL ~ (- 1)) were significantly decreased at T+ _ 2T _ (2) T _ (3) compared with those in IR group (P _ (0.05)) and T _ (2) T _ (+ +) (P _ (0.05) P _ (0.05)). There was no significant difference in serum IL-1 尾 between the two groups at T _ 2T _ 3. There was no significant difference in serum IL-1 尾 between the two groups. There was no significant difference in serum TNF- 伪 between the two groups at T _ 2T _ 3 and T _ 2T _ 3 compared with the IR group, respectively. There was no significant difference in serum TNF- 伪 between the Iso group and the IR group at the time of T _ 2T _ 3. MDA in liver tissue was significantly lower than that in T _ 3. Compared with IR group, the GR of liver tissue in Iso group was significantly higher than that in IR group, and the content of ATP in liver tissue of P0.05Sev group was significantly higher than that of IR group at T3 time point. The difference was statistically significant (P 0.05), and there was no significant difference between the two groups in the pathological changes of the liver and the corresponding changes of ischemia in the Sev group and the Iso group. Conclusion Pretreatment with sevoflurane and isoflurane can attenuate the hepatic ischemia-reperfusion injury and improve the energy metabolism of the liver.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363

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