microRNA-7对小鼠肠系膜淋巴结αβT淋巴细胞组成的影响

发布时间：2018-03-10 00:04

本文选题：miR-基因敲减　切入点：肠系膜淋巴结　出处：《中国免疫学杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的:研究miR-7(MicroRNA-7,miRNA-7)对小鼠肠系膜淋巴结αβT淋巴细胞比例的影响并初步探讨其意义。方法:利用miR-7基因敲减(Knockdown,KD)小鼠模型,观察其肠系膜淋巴结大小、重量和淋巴结细胞总数,以及HE染色的病理学变化;流式细胞术(FACS)检测淋巴结中αβT淋巴细胞亚群(CD3+T细胞、CD4+T细胞和CD8+T细胞)以及CD4+T细胞和CD8+T细胞的活化相关分子CD69、CD62L和功能相关因子IFN-γ表达的情况。结果:与野生型(Wild type,WT)小鼠相比,miR-7KD小鼠肠系膜淋巴结大小、重量指数和淋巴结细胞总数均显著增高(P0.05),且HE染色显示MLNs有病理性改变;肠系膜淋巴结中αβCD3+T细胞比例增加显著,其中以CD4+T细胞比例变化最为明显(P0.05),而CD19+B淋巴细胞比例明显下调(P0.05),但各亚群细胞总数均明显增加(P0.05);最后,CD4+T细胞和CD8+T细胞中的CD62L+细胞的比例均显著下调(P0.05),而CD69+细胞和IFN-γ+细胞比例均显著上调(P0.05)。结论:miR-7敲减后可显著影响肠系膜淋巴结中αβT淋巴细胞的组成比例,提示其对肠系膜淋巴结中淋巴细胞的组成和功能具有重要的调控作用。
[Abstract]:Objective: to study the effect of miR-7 microRNA-7 miRNA-7 on the percentage of 伪尾 T lymphocytes in mesenteric lymph nodes of mice and its significance. Methods: the size, weight and total number of mesenteric lymph nodes were observed by using miR-7 gene knockdown KD- mouse model. And pathological changes of HE staining; Flow cytometry (FACS) was used to detect the expression of activation molecules CD69, CD62L and IFN- 纬 in 伪尾 T lymphocyte subsets (CD 4 T cells and CD8 T cells), CD4 T cells and CD8 T cells in lymph nodes. The mesenteric lymph nodes of wild type WT-mice were larger than those of miR-7KD mice. The weight index and the total number of lymph node cells were significantly increased, and HE staining showed that MLNs had pathological changes, and the percentage of 伪尾 CD3 T cells in mesenteric lymph nodes increased significantly, and the percentage of 伪尾 CD3 T cells in mesenteric lymph nodes increased significantly. The percentage of CD4 T cells was the most obvious, while the proportion of CD19 B lymphocytes was down-regulated, but the total number of cells in each subgroup increased significantly. Finally, the percentage of CD62L cells in CD4 T cells and CD8 T cells decreased significantly, while the percentage of CD62L cells in CD8 T cells decreased significantly. The proportion of CD69 cells and IFN- 纬 cells were significantly up-regulated (P 0.05). Conclusion the proportion of 伪尾 T lymphocytes in mesenteric lymph nodes can be significantly affected by the knockout of miR-7. The results suggest that it plays an important role in regulating the composition and function of lymphocytes in mesenteric lymph nodes.
【作者单位】：遵义医学院免疫学教研室暨贵州省免疫学研究生教育创新基地;遵义医学院医学物理学教研室;
【基金】：国家自然科学基金(No.31370918) 教育部“新世纪优秀人才计划”项目(NCET-12-0661)资助
【分类号】：R392

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