联合应用MAGE-1与IL-18基因疫苗抗肝癌免疫治疗作用的实验研究

发布时间：2018-03-10 08:36

本文选题：肿瘤疫苗　切入点：MAGE-1　出处：《吉林大学》2010年博士论文　论文类型：学位论文

【摘要】： 本研究利用分子生物学、免疫学技术构建重组基因疫苗pcDNA3- MAGE-1和pcDNA3-hIL-18(小鼠pcDNA3-mIL-18由本实验室保存);探讨联合应用基因疫苗体内、外抗肝癌免疫治疗的作用。基因疫苗分组免疫实验小鼠,在体外验证疫苗免疫后的小鼠脾细胞对MAGE-1阳性和阴性肝癌细胞株的杀伤作用;同时我们探讨不同剂量pcIL-18在与pcMAGE-1联合应用时达到最佳杀伤效应的免疫剂量。将MAGE-1转染至小鼠肝癌Hepa1-6细胞,获得稳定表达MAGE-1基因的小鼠肝癌Hepa1-6-MAGE-1细胞系,为本研究以及进一步研究MAGE-1在肿瘤生物治疗中的应用奠定基础。体内抗肝癌的实验研究,各实验组小鼠先皮下注射Hepa1-6-MAGE-1细胞和Hepa1-6细胞再接种基因疫苗。观察不同基因疫苗对MAGE-1(+)和MAGE-1(-)荷瘤小鼠肿瘤生长和生存时间的影响。本研究结果表明MAGE-1与较小剂量IL-18的联合应用具有明显的抗肝癌免疫治疗作用。
[Abstract]:The purpose of this study was to construct recombinant gene vaccine pcDNA3- MAGE-1 and pcDNA3-hIL-18 by molecular biology and immunology. The killing effect of spleen cells of mice immunized with vaccine on MAGE-1 positive and negative hepatoma cell lines was verified in vitro. At the same time, we studied the optimal dose of pcIL-18 in combination with pcMAGE-1. MAGE-1 was transfected into mouse hepatoma Hepa1-6 cells to obtain murine hepatocarcinoma Hepa1-6-MAGE-1 cell line which expressed MAGE-1 gene stably. For this study and further study of the application of MAGE-1 in tumor biotherapy. Experimental study of anti-hepatoma in vivo, Mice in each experimental group were injected subcutaneously with Hepa1-6-MAGE-1 cells and Hepa1-6 cells and then inoculated with gene vaccine. The effects of different gene vaccines on tumor growth and survival time of mice bearing MAGE-1 () and MAGE-1-) were observed. The combined use of the drug has obvious anti-hepatoma immunotherapy effect.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R392.1

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