黄芩苷联合氟康唑对白念珠菌生物膜的抑制作用研究

发布时间：2018-03-10 08:39

本文选题：黄芩苷　切入点：白念珠菌　出处：《中国真菌学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的探讨中药有效成分黄芩苷(baicalin,BA)联合氟康唑(fluconazole,FLC)对白念珠菌(Candida albicans,C.albicans)生物膜的抑制作用。方法通过棋盘法考察BA联合FLC对白念珠菌浮游菌与生物膜的部分抑菌浓度指数(FICI);通过时间-杀菌曲线检测两药联合对白念珠菌标准株(C.albicans SC5314)的杀菌作用;以XTT减低法和干重法检测两药联合对白念珠菌SC5314生物膜代谢及生物量的影响;采用扫描电镜(Scanning electron microscopy,SEM)和激光共聚焦显微镜(Confocal laser scanning microscopy,CLSM)观察两药联合对白念珠菌SC5314生物膜形态结构的影响;以水-烃法检测两药联合对白念珠菌SC5314生物膜细胞表面疏水性(cell surface hydrophobicity,CSH)的影响;通过实时荧光定量PCR(quantitative real time PCR,qRT-PCR)检测两药联合对白念珠菌生物膜和CSH相关基因表达的影响。结果黄芩苷与氟康唑联用抗白念珠菌浮游菌的FICI介于0.28~0.75之间,对生物膜的FICI介于0.16~0.5之间,表现为协同作用;SEM和CLSM在生物膜结构上验证了两药的协同效果;两药联合可降低生物膜表面疏水性,以及使ALS1、ALS3、EAP1、SUN41和CSH1分别下调6%、51%、24%、13%和39%。结论黄芩苷具有协同氟康唑抗白念珠菌生物膜作用。
[Abstract]:Objective to investigate the inhibitory effect of baicalin in combination with fluconazolein (FLC) on Candida albicans C. albicans biofilm. Methods the inhibitory concentration index of BA combined with FLC on phytoplankton and biofilm of Candida albicans was investigated by checkerboard method. The bactericidal effect of the two drugs on C. albicans SC5314, a standard strain of Candida albicans, was detected by time-sterilization curve. The effects of the two drugs on the metabolism and biomass of SC5314 biofilm of Candida albicans were detected by XTT reduction and dry weight method. The effects of the two drugs on the morphology of SC5314 biofilm were observed by scanning electron microscope (SEM) and confocal laser scanning microscopy (electron). The effect of the two drugs on the surface hydrophobicity of Candida albicans SC5314 biofilm cells was detected by water-hydrocarbon method. The effect of the two drugs on the expression of biofilm and CSH related genes in Candida albicans was detected by real-time PCR(quantitative real time PCRQ RT-PCR.Results the FICI of baicalin and fluconazole against Candida albicans phytoplankton was between 0.28 and 0.75, and the FICI of biofilm was between 0.160.5.Results. The synergistic effect of CLSM and CLSM on the structure of biofilm was demonstrated, and the hydrophobicity of biofilm was decreased by the combination of two drugs. The results showed that SUN41 and CSH1 were down-regulated by 61% and 39%, respectively. Conclusion baicalin has synergistic effect on Candida albicans biofilm.
【作者单位】：安徽中医药大学中西医结合临床学院;安徽省中医药科学院中西医结合研究所;
【基金】：安徽省自然科学基金(1408085MH165) 安徽中医药大学校级科研项目(2014zr026)
【分类号】：R379.4

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