糖尿病心肌病动物模型的建立（英文）

发布时间：2018-03-11 21:17

本文选题：糖尿病心肌病　切入点：链脲菌素　出处：《中国组织工程研究》2015年27期 　论文类型：期刊论文

【摘要】：背景:糖尿病性心肌病是导致糖尿病患者死亡率增加的重要因素,是一种严重的糖尿病并发症。因此为研究糖尿病心肌病的发病机制及诊疗提供有效的实验动物模型显得尤为重要。目的:探索关于构建Wistar大鼠糖尿病心肌病动物模型的方法。方法:将40只大鼠随机分为对照组(n=10)和糖尿病心肌病组(n=30)。糖尿病心肌病组应用链脲菌素60 mg/kg一次性腹腔注射法构建糖尿病心肌病大鼠模型;对照组大鼠给予等体积的枸橼酸缓冲液。两组大鼠均以非高脂高糖的普通饲料喂养。结果与结论:与对照组相比,糖尿病心肌病组大鼠在腹腔注射链脲菌素3周后血糖显著升高,心肌细胞排列紊乱,细胞核大小不规则,心肌组织内胶原含量明显增多且胶原纤维粗大排列紊乱,同时反映糖尿病心肌病心肌纤维化的转化生长因子β1和Ⅰ型胶原的mR NA和蛋白表达水平亦明显增高。说明一次性腹腔注射大剂量的链脲菌素(60 mg/kg)并给予非高脂高糖的普通饲料喂养,可以构建稳定的1型糖尿病心肌病模型,方法安全有效,可行性高。
[Abstract]:Background: diabetic cardiomyopathy is an important factor in increasing mortality in patients with diabetes. It is very important to provide an effective experimental animal model for studying the pathogenesis and diagnosis and treatment of diabetic cardiomyopathy. Objective: to explore the construction of diabetic cardiomyopathy in Wistar rats. Methods: forty rats were randomly divided into control group (n = 10) and diabetic cardiomyopathy group (n = 30). Diabetic cardiomyopathy rats were induced by streptozotocin 60 mg/kg intraperitoneal injection. Rats in the control group were given citric acid buffer of equal volume. The rats in both groups were fed with normal diet without high fat and high sugar. Results and conclusion: compared with the control group, the rats in the control group were fed with the same amount of citric acid buffer. After intraperitoneal injection of streptozotocin for 3 weeks, the diabetic cardiomyopathy rats had significantly increased blood glucose, disordered arrangement of cardiomyocytes, irregular nuclear size, increased collagen content in myocardial tissue and disorder in the coarse arrangement of collagen fibers. The expression levels of mRNA and protein of transforming growth factor 尾 1 and type I collagen in diabetic cardiomyopathy myocardial fibrosis were also significantly increased, indicating that a large dose of streptozotocin 60 mg / kg was injected intraperitoneally and given non-hyperlipidemia. Sugar feed, A stable type 1 diabetic cardiomyopathy model can be constructed, and the method is safe and effective.
【作者单位】：青岛大学医学院;青岛大学医学院附属青岛市市立医院细胞移植实验室;青岛大学医学院附属青岛市市立医院心内科;青岛大学医学院附属青岛市市立医院临床检验科;
【基金】：the National Natural Science Foundation of China,No.81200175 the Natural Science Foundation of Qingdao City of China,No.13-1-4-140-jch~~
【分类号】：R587.2;R-332

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