针刺、艾灸对AA模型大鼠痛阈及“足三里”穴位局部组织液中钾离子的不同影响

发布时间：2018-03-11 21:29

本文选题：针灸　切入点：痛阈　出处：《成都中医药大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：研究针灸对AA模型大鼠痛阈以及“足三里”穴位局部组织液中钾离子浓度的影响,以期从穴位局部相关物质变化这个角度出发,探索针刺与艾灸对痛阈与穴位局部组织液中钾离子浓度的不同影响,同时也为后期研究针灸穴位局部机制提供新的实验依据。方法：以AA模型大鼠为炎性疼痛的研究载体,一批大鼠针灸“足三里”30min,用Von Frey测痛仪检测针灸前,针灸中及针灸后2h痛阈变化,另一批大鼠针灸“足三里”30min,同时采用微透析仪于“足三里”穴位局部收集针灸前,针灸中及针灸后2h组织液,每30min收集一个样品,将所得样品即时用电解质分析仪检测胞外钾离子浓度。统计分析组内比较用重复测量方差分析法,组间比较采用T检验法,P0.05表示有统计学差异。结果：针刺及艾灸后,AA模型大鼠痛阈高于针灸前(p0.05),“足三里”穴位局部组织液中钾离子浓度也高于针灸前(p0.05),空针组痛阈高于空灸组(p0.05),模灸组痛阈高于模针组(p0.05),空灸组穴位局部胞外钾离子浓度高于空针组(p0.05),模灸组穴位局部胞外钾离子浓度高于模针组(p0.05)。结论：针灸能提高AA模型大鼠痛阈；针刺和艾灸对痛阈和穴位局部胞外钾离子浓度的影响有细微区别。对正常大鼠,针刺组痛阈高于艾灸组,而艾灸对穴位局部胞外钾离子的升高作用,高峰到达所用时间短。对AA模型大鼠,艾灸组痛阈高于针刺组,并且艾灸后痛阈高峰维持时间较长,艾灸对于胞外钾离子的升高作用,维持高浓度时间也较长。
[Abstract]:Objective: to study the effect of acupuncture and moxibustion on pain threshold and potassium ion concentration in local tissue fluid of "Zusanli" acupoint in AA model rats. To explore the different effects of acupuncture and moxibustion on the concentration of potassium ions in pain threshold and local tissue fluid of acupoints, and to provide a new experimental basis for the later study of local mechanism of acupuncture and moxibustion. Methods: AA model rats were used as the research carrier of inflammatory pain. A batch of rats were treated with acupuncture and moxibustion "Zusanli" for 30 minutes. The pain threshold before acupuncture, during acupuncture and 2 hours after acupuncture were detected by Von Frey. Another group of rats received acupuncture and moxibustion "Zusanli" for 30 mins. At the same time, a sample was collected every 30 minutes before acupuncture, 2 hours after acupuncture and 2 hours after acupuncture and moxibustion, using microdialysis apparatus at Zusanli acupoint. The concentration of extracellular potassium ion was detected by electrolyte analyzer immediately. The statistical analysis group was compared with repeated measurement variance analysis. Results: the pain threshold of AA model rats after acupuncture and moxibustion was higher than that before acupuncture, the concentration of potassium ion in local tissue fluid of Zusanli acupoint was also higher than that before acupuncture, the pain threshold of empty acupuncture group was higher than that of empty moxibustion group, the pain threshold of model moxibustion group was higher than that of model acupuncture group, and the pain threshold of model moxibustion group was higher than that of model acupuncture group. The concentration of extracellular potassium ion at acupoints in moxibustion group was higher than that in empty acupuncture group, and the concentration of extracellular potassium ion in moxibustion group was higher than that in model acupuncture group. Conclusion: acupuncture can increase pain threshold of AA model rats. The effects of acupuncture and moxibustion on pain threshold and local extracellular potassium concentration at acupoints were slightly different. For normal rats, the pain threshold of acupuncture group was higher than that of moxibustion group, while moxibustion could increase the level of extracellular potassium at acupoints. For AA model rats, the pain threshold of moxibustion group was higher than that of acupuncture group, and the time of maintaining the peak of pain threshold after moxibustion was longer, and the effect of moxibustion on the increase of extracellular potassium ion and the duration of high concentration of moxibustion were also longer.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R245;R-332

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