应用微列阵技术快速鉴定常见致病性分枝杆菌

发布时间：2018-03-12 20:31

本文选题：分枝杆菌　切入点：菌种鉴定　出处：《苏州大学》2010年硕士论文　论文类型：学位论文

【摘要】： 【目的】利用微列阵技术建立一种简便、快速、灵敏、特异的分支杆菌菌种鉴定方法,为临床医师正确诊断提供依据。【方法】以DNA测序法为对照,通过微列阵技术分析20种分枝杆菌标准株及486株分枝杆菌临床分离株。【结果】应用微列阵技术分析,20种分枝杆菌标准株鉴定结果的特异性为100%,486株分枝杆菌临床分离株中,显示358株临床分离株与分枝杆菌属探针IC和结核分枝杆菌复合群探针杂交阳性,鉴定为结核分枝杆菌复合群(MTC)。128株为非结核分枝杆菌(NTM),其中47株为龟分枝杆菌龟亚种和脓肿亚种,32株为胞内分枝杆菌,15株为鸟分枝杆菌株,11株为堪萨斯分枝杆菌,4株为偶然分枝杆菌,4株为耻垢分枝杆菌,4株为海或溃疡分枝杆菌,3株为苏加尔分枝杆菌或玛尔摩分枝杆菌,2株为戈登分枝杆菌,2株为土分枝杆菌,1株为瘰疬分枝杆菌,1株为不产色分枝杆菌,2株只与分枝杆菌属探针杂交,经测序显示1株为副瘰疬分枝杆菌,1株为莫娜分枝杆菌,芯片上无鉴定这2个菌种的探针。【结论】微列阵技术是一种准确、高效的快速鉴定分枝杆菌菌种的分子诊断技术。
[Abstract]:[objective] to establish a simple, rapid, sensitive and specific method for the identification of mycobacteria by using microarray technique. [methods] 20 standard mycobacterium strains and 486 clinical isolates of Mycobacterium were analyzed by microarray technique using DNA sequencing method as control. [results] the specificity of the identification of 20 standard strains of Mycobacterium by microarray technique was 100 and 486. The results showed that 358 clinical isolates were positive for hybridization with IC and Mycobacterium tuberculosis complex probes. Identified as Mycobacterium tuberculosis complex MTCN. 128 strains as non-tuberculous mycobacteria NTMN, of which 47 strains are Mycobacterium tortoise subspecies and 32 strains of abscess subspecies, 15 strains of Mycobacterium endogenes, 15 strains of Mycobacterium avium, 11 strains of Mycobacterium Kansan, 11 strains of Mycobacterium Kansas. 4 strains of Mycobacterium fortuitum, 4 strains of Mycobacterium smearus, 4 strains of Mycobacterium ulcerans, 3 strains of Mycobacterium thuringiensis or 2 strains of Mycobacterium malmo, 2 strains of Mycobacterium Gordon, 2 strains of Mycobacterium tumefaciens, 1 strain of Mycobacterium scrofula, 1 strain of Mycobacterium scrofulla. One strain of Mycobacterium, one of which is a non-chromogenic mycobacterium, only hybridized with a probe of the genus Mycobacterium. Sequencing showed that one strain was Mycobacterium parascrofula and one strain was Mycobacterium mona. There was no probe on the microarray to identify the two strains. [conclusion] Microarray technique is an accurate and efficient molecular diagnostic technique for the identification of Mycobacterium species.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R378

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