脉冲磁场对皮层神经元离子通道的影响

发布时间：2018-03-13 00:38

本文选题：神经元　切入点：脉冲磁场　出处：《天津大学》2008年硕士论文　论文类型：学位论文

【摘要】： 随着现代工业的发展,磁场对人体的影响日趋广泛且不明确,同时也有越来越多的磁疗仪器产生,并且已应用于临床治疗,但其治疗机理尚不清楚,有待于进一步探明。因此研究磁场对细胞的作用具有现实的重要意义。本文首次从细胞层次上观察了在低强度低频脉冲磁场环境下,小鼠皮层神经元电压门控钠钾离子通道电生理特性的变化。采用频率为15Hz、强度为1.4mT的低强度低频脉冲磁场对小鼠的脑皮层神经元进行刺激,而后应用全细胞膜片钳技术测量其钠钾离子通道特性。结果显示:低强度低频脉冲磁场可使神经元电压门控离子通道特性改变,可显著影响皮层神经元离子通道激活电位和通道电流,对INa、IA、IK的作用具有电压依赖性。脉冲磁场作用于小鼠皮层神经元对钠通道峰值电流INa起到明显的抑制作用,并且其改变了钠通道的激活特性与失活特性,从而影响动作电位的去极化与超极化过程,进而会引起神经元细胞生理功能发生变化。同时脉冲磁场对瞬时外向钾电流IA和延迟整流外向钾电流IK有显著的抑制作用,脉冲磁场作用可显著地影响外向钾通道的激活与失活过程,可以改变其瞬时外向钾通道特性,调节神经元的生理功能,可能有利于受损神经元的恢复。应用膜片钳技术在细胞和分子水平探讨低强度低频脉冲磁场的生物刺激效应是一个全新的方法,本文从离子通道角度上研究脉冲磁场对脑皮层神经元的影响,为全面深入地探索电磁场的生物效应提供了借鉴。
[Abstract]:With the development of modern industry, the influence of magnetic field on human body is becoming more and more extensive and unclear. At the same time, more and more magnetic therapy instruments have been produced, and have been used in clinical treatment, but its therapeutic mechanism is not clear. Therefore, it is important to study the effect of magnetic field on cells. In this paper, we have for the first time observed the low intensity low-frequency pulsed magnetic field at the cellular level. Changes of electrophysiological characteristics of voltage-gated sodium and potassium channels in mouse cortical neurons stimulation of mouse cortical neurons by low-intensity low-frequency pulsed magnetic field with frequency of 15 Hz and intensity of 1.4 Mt. The characteristics of sodium and potassium channels were measured by whole-cell patch clamp technique. The results show that low-intensity low-frequency pulsed magnetic field can change the characteristics of voltage-gated ion channels of neurons. It can significantly affect the ion channel activation potential and channel current of cortical neurons, and has a voltage-dependent effect on INAIAIK. The effect of pulsed magnetic field on the peak sodium channel current (INa) in cortical neurons of mice was significantly inhibited. Moreover, it changes the activation and inactivation characteristics of sodium channel, thus affecting the depolarization and hyperpolarization of action potential. At the same time, pulse magnetic field can inhibit transient outward potassium current (IA) and delayed rectifier outward potassium current (IK). The action of pulsed magnetic field can significantly affect the activation and inactivation of outward potassium channel, change its transient outward potassium channel characteristics, regulate the physiological function of neurons, and may be beneficial to the recovery of injured neurons. It is a new method to investigate the biological stimulation effect of low-intensity low-frequency pulsed magnetic field at cell and molecular level by using patch clamp technique. In this paper, the effect of pulsed magnetic field on cortical neurons was studied from the angle of ion channel. It provides a reference for exploring the biological effects of electromagnetic field.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R312

【引证文献】