B族链球菌多糖—蛋白质结合物黏膜免疫小鼠血清IgG研究

发布时间：2018-03-21 10:51

本文选题：B族链球菌　切入点：多糖蛋白质结合物　出处：《兰州大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的应用B族链球菌荚膜多糖-破伤风类毒素结合物(GBS-CPS-TT)及B族链球菌荚膜多糖-白喉类毒素结合物(GBS-CPS-DT),经不同免疫途径免疫小鼠,分别测定小鼠血清中特异性IgG的抗体滴度,观察并比较两种不同载体的B族链球菌荚膜多糖-蛋白质结合物经黏膜途径诱导的全身特异性免疫反应状况,探索最佳黏膜免疫途径及免疫剂量。方法选取体重14-16g健康清洁级NIH品系雌性小鼠300只,随机取240只分别以GBS-CPS-TT、GBS-CPS-DT免疫,分为皮下注射5μg组、滴鼻5μg组、滴鼻10μg组和灌胃10μg组共8组,每组各30只。各组均免疫3次,每次免疫间隔时间2周,免疫后1周时采血,分离血清,低温保存。采用间接酶联免疫实验(ELISA)检测小鼠血清中特异性IgG的抗体滴度。结果GBS-CPS-TT、GBS-CPS-DT结合物滴鼻免疫、灌胃免疫和皮下注射均可以诱导产生特异性血清IgG抗体。滴鼻10μg组诱导产生的IgG抗体滴度明显高于滴鼻5μg组(P0.01)；当免疫剂量相同时,滴鼻免疫诱导产生的IgG抗体滴度显著低于皮下注射产生的IgG抗体滴度(P0.01)。作为载体蛋白TT与DT比较无差异(P0.05)。对照组未检出特异性IgG抗体。结论GBS-CPS-TT、GBS-CPS-TT结合物经滴鼻免疫小鼠后可产生明显的系统性免疫应答,滴鼻10μg组的免疫效果优于5μg组,抗体滴度有显著性差异意义,表明较大的免疫剂量达到的免疫效果可能更优；黏膜免疫后第二、三针诱导应答产生的抗体滴度具有明显的递增增强效应,表明结合物有免疫记忆及加强免疫应答效应；GBS多糖蛋白质结合物的黏膜免疫机制还有待进一步研究。
[Abstract]:Objective to immunize mice with group B streptococcus perfringens polysaccharide tetanus toxoid conjugate (GBS-CPS-TTT) and group B streptococcus perfringens polysaccharide diphtheria toxoid conjugate (GBS-CPS-DTT), and determine the antibody titers of specific IgG in serum of mice. To observe and compare the systemic specific immune response induced by mucosal pathway of two different carriers of streptococcus B group capsule polysaccharides and protein conjugates, and to explore the best mucosal immune pathway and immune dose. Methods A total of 300 healthy and clean grade NIH strains of female mice weighing 14-16 g were selected and immunized with GBS-CPS-CPS-DT. 240 mice were divided into 5 渭 g subcutaneous injection group, 5 渭 g nasal drip group, 10 渭 g dripping nose group and 10 渭 g gavage group with 30 rats in each group. The blood samples were collected at 1 week after immunization, the serum was isolated and preserved at low temperature. The antibody titers of specific IgG in serum of mice were detected by indirect enzyme-linked immunosorbent assay (Elisa). Results the specific serum IgG antibody was induced by nasal immunization with GBS-CPS-TT conjugate GBS-CPS-DT, intragastric immunization and subcutaneous injection. The titer of IgG antibody in 10 渭 g group was significantly higher than that in 5 渭 g group, and the titer of IgG antibody was significantly higher in 10 渭 g group than that in 5 渭 g group. The titer of IgG antibody induced by nasal immunization was significantly lower than that produced by subcutaneous injection of IgG antibody (P 0.01). There was no difference between TT and DT as carrier protein (P 0.05). No specific IgG antibody was detected in the control group. Conclusion GBS-CPS-TTS-TT conjugate can produce obvious systemic immune response after nasal drip immunization in mice. The immune effect of 10 渭 g group is better than that of 5 渭 g group, and there is significant difference in antibody titer, which indicates that the immune effect reached by larger immune dose may be better than that in 5 渭 g group. The titer of antibody induced by three needles was significantly increased after mucosal immunization, which indicated that the conjugate had immune memory and enhanced immune response. The mucosal immune mechanism of GBS polysaccharide protein conjugate was still to be further studied.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R392

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