阴道滴虫氢化酶体蛋白蛋白的预测及其导肽的进化分析

发布时间：2018-03-22 01:35

本文选题：导肽　切入点：线粒体　出处：《浙江大学》2009年博士论文　论文类型：学位论文

【摘要】：氢化酶体是厌氧原虫或真菌细胞内的亚细胞结构,可通过丙酮酸代谢产ATP和分子氢,现有的研究认为氢化酶体与线粒体具有共同的起源。蛋白组分的确定对深入了解质体的生化代谢和进化过程有重要的意义。虽然目前已有多种分子生物学、蛋白组学和计算预测的方法应用于线粒体蛋白的定位分析中,但氢化酶体相关领域的研究进展却相对缓慢。本文在氢化酶体和线粒体进化关系和蛋白转运机制的研究基础上,通过归纳线粒体蛋白与氢化酶体蛋白导肽共有的生物学特征,运用简单规则和特异性模式构建与搜索的方法,对阴道滴虫的氢化酶体蛋白进行预测,为进一步通过实验方法鉴定氢化酶体蛋白提供了有效的候选蛋白。应用本文提出的HdPred方法共预测获得70个可能的氢化酶体蛋白,对该结果的深入评价发现,其中50%的组分通过实验方法或在其它预测方法得到了确认,表明该方法能较为有效地对氢化酶体蛋白进行富集。本文的研究报道了多个能量代谢相关的氢化酶体蛋白,进一步说明了氢化酶体在能量代谢过程中的重要作用,同时本文的方法发现氢化酶体中可能具有的脂肪酸代谢相关的组分,为全面了解该质体的生化代谢途径提供了明确的目标基因。此外,通过比较不同方法对导肽序列的预测结果,显示本文采用的方法在导肽序列的预测上具有更高的准确性。本文首次全面地对线粒体蛋白导肽的起源和进化方式进行归纳,并按照其起源方式分为外源性获得和自身起源两个类型。在蛋白质组学对阴道滴虫的氢化酶体蛋白鉴定结果的基础上,本文对其中Hsp60蛋白的导肽的起源和进化进行了探讨,分析表明其导肽可能通过基因水平上的复制获得,从而对现有关于导肽起源的研究进行了有益的补充。
[Abstract]:Hydrogenosome is anaerobic protozoa or fungi subcellular structures within the cell, through the pyruvate metabolism and molecular hydrogen production of ATP, the existing research that the hydrogenosomes and mitochondria had a common origin. Protein components of certain understanding has important significance of biochemical metabolism and evolution of plastids. Although there have been a variety of molecular biology location analysis, proteomics and computational prediction method is applied to the mitochondrial protein, but the research progress of hydrogenosomes in related fields is relatively slow. In this paper, based on the transfer mechanism in hydrogenosomes and evolutionary relationship of the mitochondrial protein and, through the induction of mitochondrial protein and hydrogenase protein peptide common biologic guide the characteristics, use simple rules and specific mode and search method of hydrogenosomes protein on Trichomonas vaginalis predicted by experimental method for further reference The hydrogenated enzyme protein provides an effective candidate protein.
HdPred using the method presented in this paper is obtained to predict the 70 possible hydrogenosomes protein, found in-depth evaluation of the results, of which 50% of the components by experimental methods or other prediction methods in the confirmation, show that this method can be effective to hydrogenosomes in protein enrichment. This research reports a plurality of energy metabolism related protein hydrogenosomes, further demonstrated the important role of hydrogenosomes in energy metabolism, and this method may have found hydrogenosomes in fatty acid metabolism related components, provides a clear target gene to understand the biochemical metabolic pathways of the body. In addition, through the comparison of different methods to guide peptide sequence prediction. The results show this method has higher accuracy in predicting the guide peptide sequence.
For the first time full of mitochondrial origin and evolution of peptides were summarized, and according to their mode of origin is divided into exogenous and its origin two types. Based on vaginal trichomonad hydrogenosomes protein identification results in proteomics, the origin and evolution of leader peptide of Hsp60 protein has carried on the discussion, analysis showed that the peptide may guide through genetic level replication, which is a useful supplement to the existing research on the origin of the guide peptide.

【学位授予单位】：浙江大学
【学位级别】：博士
【学位授予年份】：2009
【分类号】：R341

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