豚鼠膀胱Cajal样间质细胞的分布及意义

发布时间：2018-03-25 06:35

本文选题：ICCs样细胞　切入点：KIT蛋白　出处：《石河子大学》2009年硕士论文

【摘要】：目的:本课题以正常成年豚鼠为研究对象,通过对膀胱组织内Cajal样间质细胞(interstitial cells of Cajal, ICCs)的分布情况以及其与周围一氧化氮阳性神经元细胞的分布规律进行观察;对体外培养的ICCs样细胞进行分泌功能特性研究,探讨其作为膀胱的起博细胞的分布、功能及意义,为临床上治疗某些疾病(如detrusor instability,DI)提供新的切入点。方法:本实验通过豚鼠膀胱冰冻切片运用c-kit免疫荧光观察ICCs样细胞的在正常成年豚鼠膀胱组织的分布情况;结合c-kit、NOS2免疫荧光双标技术观察ICCs样细胞与一氧化氮阳性神经元的分布关系;采用酶消化法,得到体外培养状态下的ICCs样细胞,并运用c-kit、NOS2免疫荧光双染技术,探讨ICCs样细胞的分泌功能。结果:通过免疫荧光观察到ICCs样细胞在豚鼠膀胱内的分布情况:组织学分布组中肌层多于粘膜下层,粘膜下层多于粘膜层;解剖学分布组中膀胱顶多于膀胱体,膀胱体多于膀胱颈;通过荧光双标法可以观察到,ICCs样细胞分布于一氧化氮阳性神经元周围;通过免疫荧光双染体外的ICCs样细胞,可以发现c-kit、NOS2在ICCs样细胞内共表达的现象。结论:ICCs样细胞在膀胱内不同部位的分布情况不同,在膀胱顶和膀胱肌层内分布较多,与其生理功能相符合,为其作为膀胱起搏细胞打下了组织分布的基础;ICCs样细胞分布于一氧化氮阳性神经周围,两种都分布于膀胱平滑肌组织间,两者联系密切,为研究“神经- ICCs样细胞-平滑肌”作用单元提供了有力的证据;ICCs样细胞荧光双染发现其具备分泌NO的结构基础,为研究ICCs样细胞的分泌功能进而推断其对膀胱逼尿肌的作用方式提供了基础,同时为临床上治疗某些疾病开辟了新的切入点。
[Abstract]:Objective: to study the distribution of interstitial cells of Cajal cells of Cajal (ICCs) in normal adult guinea pigs, and to observe the distribution of ICCs with the surrounding nitric oxide (no) positive neurons. The secretory function of ICCs like cells cultured in vitro was studied, and the distribution, function and significance of PBCs in bladder were discussed, which provided a new entry point for the clinical treatment of some diseases such as detrusor instability. Methods: the distribution of ICCs like cells in normal adult guinea pig bladder was observed by c-kit immunofluorescence using frozen section of guinea pig bladder. The relationship between the distribution of ICCs like cells and nitric oxide positive neurons was observed by using the double immunofluorescence staining technique of c-kiti NOS2, the ICCs like cells in vitro were obtained by enzyme digestion, and the double immunofluorescence staining technique was used to detect the distribution of ICCs like cells and nitric oxide positive neurons. To investigate the secretory function of ICCs-like cells. Results: the distribution of ICCs like cells in the bladder of guinea pigs was observed by immunofluorescence: the muscular layer was more than the submucosal layer and the submucosal layer was more than the mucosal layer in the histological distribution group, and the bladder in the anatomical distribution group was more than the bladder body. The presence of ICCS-like cells around the nitric oxide positive neurons was observed by fluorescence double labeling method, and the co-expression of c-kitS2-like cells in ICCs like cells could be found by immunofluorescence double staining in vitro. Conclusion the distribution of ICCs like cells in different parts of bladder is different, and the distribution of ICCs like cells in the top of bladder and the muscular layer of bladder is more, which is consistent with its physiological function. The ICCs like cells were distributed around the nitric oxide positive nerve, and both of them were distributed in the smooth muscle tissue of the bladder, which were closely related to each other. For the study of neuro-ICC-like cell-smooth muscle unit, it provides strong evidence that ICC-like cells have the structural basis of no secretion by fluorescence double staining. It provides a basis for studying the secretory function of ICCs-like cells and inferring its mode of action on bladder detrusor. It also provides a new entry point for the clinical treatment of some diseases.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R329

【相似文献】