跑步锻炼对抑郁症模型大鼠大脑白质及白质内有髓神经纤维影响的体视学研究

发布时间：2018-03-30 18:06

本文选题：抑郁症　切入点：跑步锻炼　出处：《中国组织化学与细胞化学杂志》2015年01期

【摘要】：目的运用无偏体视学方法定量研究慢性不可预见性应激(CUS)所致的抑郁模型大鼠大脑白质及白质内有髓神经纤维的改变及跑步锻炼对其改变的影响。方法雄性SD大鼠,适应环境1w,筛选出符合条件的40只大鼠将其随机分为空白对照组(10只)和抑郁模型组(30只)。从第2w开始模型组动物采用慢性不可预见性应激的方法建立抑郁症大鼠模型,为期4w。选取建模成功的大鼠20只,随机等分为模型对照组和模型跑步组,模型跑步组大鼠进行4w的跑步锻炼。采用糖水偏好试验、强迫游泳试验及旷场试验进行行为学检测;利用体视学方法测定白质的总体积及白质内有髓神经纤维的总长度。结果应激第4w末,与空白对照组相比,抑郁模型组大鼠体重、糖水偏好百分比、旷场得分均降低,强迫游泳不动时间增加,差异具有统计学意义(P0.05);跑步锻炼四周后,模型跑步组糖水偏好百分比显著性高于模型对照组(P0.05)。模型对照组大鼠大脑白质总体积、有髓神经纤维总长度均显著性低于空白对照组(P0.01);与模型对照组相比,模型跑步组大鼠大脑白质总体积及有髓神经纤维总长度显著性增加(P0.05);与空白对照组相比,模型跑步组大鼠大脑白质总体积无显著性差异(P0.05),白质内有髓神经纤维总长度显著性降低(P0.05)。结论跑步锻炼对抑郁症模型大鼠大脑白质内有髓神经纤维改变具有积极保护作用,这可能是跑步锻炼治疗抑郁症的重要结构基础之一。
[Abstract]:Objective to study quantitatively the changes of myelinated nerve fibers in the white matter and white matter and the effect of running exercise on the changes of myelinated nerve fibers in the white matter and white matter of the depression model rats induced by chronic unpredictable stress (CUSS) by using unbiased stereological method. Methods male SD rats were used to study the changes of myelinated nerve fibers in the white matter and white matter. After 1 week of adaptation, 40 eligible rats were randomly divided into blank control group (n = 10) and depression model group (n = 30). From the second week, the model group was established by chronic unpredictable stress. For 4 weeks, 20 rats were randomly divided into two groups: model control group and model running group. The rats in the model group underwent 4 weeks of running exercise. The behavioral tests were performed by sugar water preference test, forced swimming test and open field test. The total volume of white matter and the total length of myelinated nerve fibers in white matter were measured by stereological method. Results at the end of the 4th week of stress, compared with the blank control group, the weight, the percentage of sugar and water preference and the open field score of the depression model group were all lower than those of the blank control group. After four weeks of running, the percentage of sugar water preference in the model group was significantly higher than that in the model control group (P 0.05). Compared with the model control group, the total volume of white matter and the total length of myelinated nerve fibers in the model running group increased significantly (P 0.05), compared with the blank control group, the total length of myelinated nerve fibers was significantly lower than that of the control group (P < 0.05). There was no significant difference in the total volume of white matter and the total length of myelinated nerve fibers in the white matter decreased significantly in the model group. Conclusion running exercise has a positive protective effect on the changes of myelinated nerve fibers in the white matter of depression rats. This may be one of the important structural foundations of running exercise in the treatment of depression.
【作者单位】：重庆医科大学基础医学院组织胚胎学教研室;重庆医科大学基础医学院干细胞与组织工程研究室;重庆医科大学基础医学院附属第一医院老年科;重庆医科大学基础医学院生理学教研室;
【基金】：国家自然科学基金资助(31300985,81171238,31271288,81301149) 重庆市科委自然科学基金资助(CSTC2011jjA10066) 重庆市首批“百名学术学科领军人才”培养计划资助(2012)
【分类号】：R749.4;R-332

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