Fogarty球囊构建兔颈总动脉损伤及移植内皮祖细胞治疗的动物模型

发布时间：2018-03-30 19:50

本文选题：Fogarty球囊　切入点：球囊损伤　出处：《东南大学学报(医学版)》2015年04期

【摘要】：目的:利用Fogarty球囊建立兔颈总动脉球囊损伤及损伤处移植内皮祖细胞(endothelial progenitor cell,EPCs)治疗的动物模型。方法:培养兔外周血中EPCs并以Fe2O3磁性纳米颗粒标记。将实验兔随机分为细胞组(12只)和空白组(10只),经股动脉入路利用Fogarty球囊损伤两组兔右侧颈总动脉,并用Fogarty球囊阻断血流后将标记EPCs移植至细胞组血管损伤段,将生理盐水注入空白组;术后7 d取2只细胞组兔损伤血管行普鲁士蓝染色;术后3 d,1、4、8周行MR扫描;术后8周取损伤血管,行HE、弹力纤维及PCNA染色。结果:术后7 d普鲁士蓝染色示损伤内膜可见含铁EPCs附着,MR扫描示术后4周空白组兔损伤血管壁稍有增厚,术后8周明显增厚,细胞组术后4周损伤血管管壁未见明显增厚,8周后稍有增厚。病理学检查示,术后8周细胞组与空白组内膜/中膜比(N/M)分别为0.34±0.05和1.14±0.10(P0.01),PCNA阳性细胞指数为0.08±0.01和0.31±0.01(P0.05)。结论:Fogarty球囊成功构建兔颈总动脉损伤狭窄模型并阻断血流实现EPCs定向移植至血管损伤处,参与内膜的修复。
[Abstract]:Objective: to establish an animal model of rabbit common carotid artery injury and injury by transplantation of endothelial progenitor cells (EPCs) with Fogarty balloon. Methods: EPCs in rabbit peripheral blood was cultured and labeled with Fe2O3 magnetic nanoparticles. Divided into cell group (n = 12) and blank group (n = 10), the right common carotid artery was injured by Fogarty balloon via femoral artery approach in two groups. Fogarty balloon was used to block the blood flow and then the labeled EPCs was transplanted into the vascular injury segment of the cell group and injected with normal saline into the blank group. The injured vessels of 2 rabbits in the cell group were taken 7 days after operation for Prussian blue staining. Results: on the 7th day after operation, Prussian blue staining showed that the injured intima could be seen to be iron-containing EPCs adherent Mr scan showed that the injured vascular wall was slightly thickened in the blank group at 4 weeks after operation, and significantly thickened at the 8th week after operation. In the cell group, there was no obvious thickening of the injured vascular wall 4 weeks after operation. At 8 weeks after operation, the ratio of intima to medial membrane in the cell group and the blank group were 0.34 卤0.05 and 1.14 卤0.10 respectively. The index of PCNA positive cells was 0.08 卤0.01 and 0.31 卤0.01P0.05 respectively. Conclusion the stenosis model of common carotid artery injury in rabbits was successfully constructed by using the: Fogarty balloon and EPCs was transplanted to the vascular injury site by blocking the blood flow. Participate in the restoration of intima.
【作者单位】：东南大学附属中大医院介入与血管外科江苏省分子影像与功能影像重点实验室;
【基金】：国家自然科学基金资助项目(81171433/H1816) 中央高校基本科研业务费专项资金资助项目(2242015K40009)
【分类号】：R543.4;R-332

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