蛋氨酸脑啡肽作为共刺激因子对树突状细胞体外培养及抗肿瘤作用的研究

发布时间：2018-03-31 11:24

本文选题：蛋氨酸脑啡肽　切入点：树突状细胞　出处：《吉林大学》2008年硕士论文

【摘要】： 树突状细胞(Dendritic cells,DC)是目前已知的功能最强的抗原递呈细胞(APC),参与抗原的识别、加工处理与递呈,是机体免疫反应的首要环节,也是免疫反应中识别自我与非我的关键环节。近年来国际上对DC的研究进展很快,对于DC在肿瘤免疫、感染免疫、移植免疫、自身免疫等方面作用的认识不断深入。但是由于DC仅占外周血单个核细胞总数的0.5%～1%,难以分离纯化;而且常规的基础DC培养基中主要成分为粒细胞-巨噬细胞集落刺激因子(GM-CSF),其虽然能促进DC细胞的成熟,但在体外大量培养和扩增以达到治疗用途,尚存在一定问题。这限制了DC的临床应用。因此,如何获得大量高纯度的成熟DC是DC研究的方向之一。蛋氨酸脑啡肽(Methionine enkephalin ,MEK)对自然杀伤细胞、单核细胞、巨噬细胞和细胞因子的释放都有调节作用,而且MEK能够增强自然杀伤细胞活性和诱导白细胞介素2、白细胞介素4的分泌。因此,本课题尝试用MEK作为共刺激因子,研究其对人外周血来源的DC细胞分化成熟的作用,探讨MEK在体外刺激DC细胞,促进其成熟的可行性。
[Abstract]:Dendritic cells (DC) is the most powerful antigen presenting cell known at present. It is involved in antigen recognition, processing and presentation. It is the most important link of immune response, and also the key link of self-recognition and non-self recognition in immune response.In recent years, there has been a rapid development in the research of DC in the world. The role of DC in tumor immunity, infection immunity, transplantation immunity, autoimmunity and so on has been deeply understood.However, since DC only accounts for 0.5% of the total number of peripheral blood mononuclear cells, it is difficult to isolate and purify, and the main component of conventional basic DC culture medium is granulocyte-macrophage colony stimulating factor (GM-CSFN), although it can promote the maturation of DC cells.However, in vitro culture and amplification to achieve therapeutic purposes, there are still some problems.This limits the clinical application of DC.Therefore, how to obtain a large number of high-purity mature DC is one of the research directions.Methionine enkephalin can regulate the release of natural killer cells, monocytes, macrophages and cytokines, and MEK can enhance the activity of natural killer cells and induce the secretion of interleukin 2 and interleukin 4.Therefore, we try to use MEK as a costimulatory factor to study the effect of MEK on the differentiation and maturation of DC cells from human peripheral blood, and to explore the feasibility of stimulating DC cells by MEK in vitro and promoting their maturation.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

【参考文献】