单因素刺激方法致大鼠肾精亏虚的动物模型及肾精亏虚对免疫功能影响的相关研究

发布时间：2018-03-31 13:29

本文选题：肾精亏虚　切入点：动物模型　出处：《成都中医药大学》2009年硕士论文

【摘要】： 研究目的：通过研究单因素刺激方法致大鼠肾精亏虚的动物模型,探讨肾精亏虚对免疫功能影响,为临床感染性疾病的防治起一定的指导作用。研究方法：基于“房劳伤肾”的理论,采用人工单因素刺激致肾精亏虚的方法,在持续的一段时间内,通过自制电刺激仪连续刺激大鼠的敏感部位使其生殖器充血勃起并排出精液,建立单因素刺激所致肾精亏虚的动物模型；造模结束后,分析天平测定大鼠睾丸湿重,HE染色法观察睾丸组织形态变化,使用放射免疫法测定大鼠血清睾酮的含量；全血细胞分析仪测定大鼠血液中红细胞和血红蛋白含量；分析天平测定大鼠胸腺和脾脏的湿重,使用酶联免疫法检测大鼠血清IgG浓度和IL-2浓度；感染肺炎克雷伯菌后,使用酶联免疫法检测大鼠血清TNF-a浓度和IL-6浓度。实验结果： (1)造模后睾丸的体积缩小,湿重显著减轻。 (2)睾丸组织呈萎缩状态：大部分曲细精管退化变性,管腔内生殖上皮变薄,各级精母细胞减少排列稀疏,精子减少甚至没有精子,间质水肿,曲细精管之间结缔组织增多,界膜增厚并纤维化透明样变性。对照组大鼠各级精母细胞排列有序,精子增多,界膜变薄,间质水肿减轻。 (3)血清睾酮的含量模型一组较正常组降低,模型二组明显高于正常组,左归丸对照组高于模型一组但低于模型二组,右归丸对照组均低于模型一组和模型二组。 (4)血液红细胞和血红蛋白含量模型一组与正常组相当,模型二组的血液红细胞和血红蛋白低于正常组,左归丸对照组和右归丸对照组高于模型二组。 (5)血清IgG浓度模型一组升高,模型二组最高,左归丸对照组较模型一组升高但低于模型二组,右归丸对照组均低于模型一组和模型二组。 (6)IL-2含量模型一组高于正常组,模型二组较正常组降低,左归丸对照组和右归丸对照组均高于模型一组和模型二组。 (7)经肺炎克雷伯菌感染后,血清TNF-a浓度正常感染组和正常组相当,模型一感染组的血清TNF-a浓度均较正常感染组降低；血清TNF-a浓度左归丸对照感染组接近正常感染组,右归丸对照感染组较正常感染组升高。 (8)经肺炎克雷伯菌感染后,血清IL-6含量正常感染组较正常组升高,模型一感染组的血清IL-6含量较正常感染组降低,左归丸对照组和右归丸对照组的血清IL-6含量均低于正常感染组。研究结论： (1)通过人工单因素刺激的方法可以建立大鼠的肾精亏虚动物模型。 (2)人工单因素刺激的方法致肾精亏虚可以导致机体免疫功能紊乱。 (3)人工单因素刺激的方法致肾精亏虚存在容易感染疾病的趋势。
[Abstract]:Objective: to investigate the effect of deficiency of kidney essence on immune function in rats with deficiency of kidney essence by single factor stimulation, and to play a guiding role in the prevention and treatment of clinical infectious diseases. Methods: based on the theory of "kidney injury caused by room strain", the method of artificial single factor stimulation was used to induce deficiency of kidney essence, which lasted for a period of time. The animal model of kidney essence deficiency caused by single factor stimulation was established by continuous stimulation of sensitive parts of the genitals of rats with congestive erection and ejection of semen by self-made electric stimulator, and the model was made after the completion of the model. The morphological changes of testis were observed by HE staining method of wet weight of testis, the content of testosterone in serum was measured by radioimmunoassay, the contents of erythrocytes and hemoglobin in blood of rats were measured by whole blood cell analyzer. The wet weight of thymus and spleen of rats was measured by balance, serum IgG and IL-2 were detected by enzyme linked immunosorbent assay (Elisa), and serum TNF-a and IL-6 were detected by Elisa after infection with Klebsiella pneumoniae (Klebsiella pneumoniae). Experimental results:. 1) the volume of testis was reduced and the wet weight was significantly reduced after modeling. (2) testicular tissue showed atrophy: most of the seminiferous tubules degenerated and denatured, the reproductive epithelium thinned in the lumen, the number of spermatocytes decreased and sparse, the spermatozoa decreased or even did not have sperm, the interstitial edema, and the connective tissue between seminiferous tubules increased. In the control group, the spermatocytes arranged orderly, the sperm increased, the boundary membrane became thinner, and the interstitial edema was alleviated. The serum testosterone content in model group 1 was lower than that in normal group, the level of serum testosterone in model group 2 was significantly higher than that in normal group, the level of serum testosterone in model group 1 was higher than that in model group 1 but lower than that in model group 2, and that in control group of Yougui pill was lower than that in model group 1 and model 2. The contents of erythrocyte and hemoglobin in the model group were similar to those in the normal group. The erythrocyte and hemoglobin in the model group 2 were lower than those in the normal group, and the contents of red blood cell and hemoglobin in the model group were higher than those in the control group and the right Gui pill group. Serum IgG concentration in model group 1 was higher than that in model group 2, the level of serum IgG in control group was higher than that in model group 1 but lower than that in model group 2, and that in control group was lower than that in model group 1 and model 2. The level of IL-2 in model group 1 was higher than that in normal group, and the level of IL-2 in model group 2 was lower than that in normal group. The level of IL-2 in model group 1 and group 2 were higher than those in group 1 and group 2. (7) after infection with Klebsiella pneumoniae, the serum TNF-a concentration in the normal infection group was similar to that in the normal group, the serum TNF-a concentration in the model first infection group was lower than that in the normal infection group, and the serum TNF-a concentration in the control group was close to that in the normal infection group. The control group of Yougui pills was higher than the normal group. After infection with Klebsiella pneumoniae, the serum IL-6 content in the normal infection group was higher than that in the normal group, and the serum IL-6 content in the model first infection group was lower than that in the normal infection group. The levels of serum IL-6 in the control group and Yougui pill group were lower than those in the normal infection group. The study concluded that:. 1) the animal model of deficiency of kidney essence can be established by artificial single factor stimulation. 2) deficiency of kidney essence caused by artificial single factor stimulation can lead to disorder of immune function. 3) deficiency of kidney essence caused by artificial single factor stimulation has the tendency of infection easily.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R-332;R229

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