磷酸铝凝胶、康复新液对溶血卵磷脂相关反流性食管炎模型大鼠食管组织学和IL-8、PGE2的影响

发布时间：2018-04-01 08:16

本文选题：反流性食管炎　切入点：溶血卵磷脂　出处：《南方医科大学学报》2015年04期

【摘要】：目的探讨磷酸铝凝胶、康复新液对溶血卵磷脂相关反流性食管炎模型大鼠食管组织学结构和组织IL-8、PGE2含量的影响及其可能的作用机制。方法 60只SD大鼠,随机分为磷酸铝组(n=10)、康复新组(n=10)、磷酸铝+康复新组(n=10)、模型组(n=20)、对照组(n=10),采用食管灌注1.5 mg/L溶血卵磷脂+0.1 mol/L HCI(含0.5%胃蛋白酶)方法制备溶血卵磷脂相关反流性食管炎模型,对照组灌注液为等量生理盐水,连续14 d,处死10只模型组大鼠和全部对照组大鼠并取出完整食管,剩余4组大鼠分别予磷酸铝凝胶、康复新液、磷酸铝凝胶+康复新液、生理盐水经食管灌注干预治疗14 d,处死所有大鼠并取出完整食管,分别检测食管组织一般形态学、超微形态学和放免法检测组织IL-8、PGE2含量。结果模型制备结束时模型组与对照组组织形态学比较:模型组一般形态学表现为粘膜及粘膜下层大量炎症细胞浸润,上皮细胞空泡变性、糜烂甚至溃疡形成,超微结构改变表现为粘膜上皮细胞呈大片脱落,对照组的一般形态学和超微结构均正常。治疗结束时各治疗组和模型组比较:各治疗组食管的一般形态学、超微结构显示粘膜损伤程度较轻,粘膜损伤指数、食管组织IL-8、PGE2含量均较模型组低(P0.05)。磷酸铝+康复新组分别与磷酸铝组、康复新组比较:食管组织的一般形态学、超微结构显示粘膜损伤程度均较轻,粘膜损伤指数、IL-8、PGE2含量均低(P0.05)。结论康复新液、磷酸铝凝胶对溶血卵磷脂与盐酸联合灌注所致反流性食管炎模型大鼠的食管组织具有保护作用,其机制可能与其降低IL-8、PGE2水平、阻止炎症发生及发展有关,溶血卵磷脂与盐酸在十二指肠胃食管反流中作为混合反流物引起GERD,其致病机制可能与上调食管组织的IL-8、PGE2的含量相关。
[Abstract]:Objective to investigate the effects of aluminophosphate gel and kangangxin solution on the histological structure and IL-8 PGE2 content of esophagus in rats with lysophosphatidylin-associated reflux esophagitis (Lecithin) and its possible mechanism.Methods 60 SD rats,The model of reflux esophagitis was established by esophageal perfusion of 1.5 mg/L lysophosphatidylcholine 0.1 mol/L HCI (containing 0.5% pepsin) in the model group and 1 5 mg/L lysophosphatidylcholine 0.1 mol/L HCI (containing 0.5% pepsin) in the model group, the model was randomly divided into three groups: aluminum phosphate group (n = 10), rehabilitation group (n = 10), aluminum phosphate group (n = 10), model group (n = 20) and control group (n = 10).The control group was perfused with the same amount of normal saline for 14 days. Ten rats in the model group and all the rats in the control group were killed and the complete esophagus was removed. The remaining 4 groups were treated with aluminophosphate gel, Kangxin solution and Kangxin solution respectively.All the rats were killed and the complete esophagus was taken out for 14 days. The general morphology of esophagus, the content of IL-8 and PGE2 in the tissues were detected by ultramorphology and radioimmunoassay.Results at the end of the model preparation, the histomorphology of the model group was compared with that of the control group. The morphological features of the model group were as follows: a large number of inflammatory cells infiltrated the mucous membrane and submucosa, vacuolar degeneration of epithelial cells, erosion and even ulceration.The ultrastructural changes were as follows: the mucosal epithelial cells were abscission, and the general morphology and ultrastructure of the control group were normal.Comparison between each treatment group and model group at the end of treatment: the general morphology and ultrastructure of the esophagus in each treatment group showed that the degree of mucosal injury was lighter, the index of mucosal injury and the content of IL-8 / PGE2 in esophageal tissue were lower than those in the model group.Compared with the aluminophosphate group, the general morphology and ultrastructure of esophagus showed that the degree of mucosal injury was lighter, and the content of IL-8 and PGE2 was lower than that of the control group.Conclusion Kangfeng Xin solution and aluminophosphate gel have protective effect on esophageal tissue of rats with reflux esophagitis induced by combined infusion of lysophosphatidylcholine and hydrochloric acid. The mechanism may be related to the decrease of IL-8 PGE2 level and the prevention of inflammation.Lysophosphatidylcholine (Lecithin) and hydrochloric acid were used as mixed reflux in duodenogastric reflux to induce GERD, which may be related to the up-regulation of IL-8 PGE2 in esophageal tissue.
【作者单位】：广西医科大学附属肿瘤医院内镜室;广西医科大学第一附属医院感染性疾病科;
【基金】：广西北海市市级立项科研项目(北科合200601067)
【分类号】：R571;R-332

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