大鼠肾移植动物模型的建立

发布时间：2018-04-02 05:17

本文选题：大鼠　切入点：模型　出处：《安徽医科大学》2009年硕士论文

【摘要】： 目的为进行移植免疫研究的需要,建立一种稳定可靠的大鼠肾移植动物模型。并探讨两种术式的优缺点。方法80只SD大鼠分两组进行,A组(供受体各20只)采用大鼠肾动静脉的端端吻合,输尿管带部分膀胱壁与受体膀胱吻合法,即原位肾移植组;B组(供受体各20只)是将供体肾动脉相连的腹主动脉及肾静脉以端侧吻合的方式移植到受体肾动脉稍下方的腹主动脉和下腔静脉。通过分析两组移植后大鼠死亡率、手术时间、及并发症发生情况以得出稳定可靠的动物模型建立方法。并了解肾动静脉吻合方式对移植大鼠的影响。结果A组20只移植大鼠平均存活21.4d,平均手术时间121.05±15.02min,术后常见的并发症主要有肾血管的狭窄和血栓、吻合口出血、输尿管坏死等。B组20大鼠平均存活24.4d,平均手术时间127.7±11.98min,术后最常见并发症为下身瘫痪、肾血管血栓、吻合口出血、输尿管狭窄或尿瘘、坏死等。A组与B组在术后各时间段的死亡率以及平均手术时间无明显差别(P0.05),A组的动脉吻合时间明显长于B组(P0.05),而静脉吻合时间小于B组(P0.05),均具有统计学意义;输尿管带部分膀胱瓣的吻合时间无显著性差异(P0.05)。结论大鼠肾动静脉端端吻合法的原位移植术能建立一种稳定可靠的动物模型,其具有并发症少,愈合快,而成功率基本相当的优点,并且供肾切取时肾静脉预留结扎线的方法使肾静脉吻合时间明显缩短。
[Abstract]:Objective in order to carry out the research of transplantation immunity, A stable and reliable animal model of renal transplantation in rats was established and the advantages and disadvantages of the two methods were discussed. Methods 80 SD rats were divided into two groups and received end-to-end anastomosis of renal arteriovenous vessels in group A (20 recipients each). Partial bladder wall of ureteral band and recipient bladder anastomosis, In orthotopic renal transplantation group B (20 recipients each), the abdominal aorta and the renal vein connected with the donor renal artery were transplanted to the abdominal aorta and the inferior vena cava slightly below the recipient renal artery by end-to-side anastomosis. Group A, the mortality rate of rats after transplantation, Operation time, In order to establish a stable and reliable animal model, and to understand the effect of renal arteriovenous anastomosis on the transplanted rats, the average survival time of 20 rats in group A was 21.4 days and the average operative time was 121.05 卤15.02 min. The main complications were renal vascular stenosis and thrombosis. The average survival time of 20 rats in group B was 24.4 days, and the average operative time was 127.7 卤11.98 min. The most common postoperative complications were lower body paralysis, renal vascular thrombosis, anastomotic bleeding, ureteral stenosis or urinary fistula. There was no significant difference in mortality and mean operative time between group A and group B after operation. The time of arterial anastomosis in group A was significantly longer than that in group B (P 0.05), but the time of venous anastomosis was less than that in group B (P 0.05). There was no significant difference in anastomosis time of partial bladder flap in ureteral band. Conclusion Orthotopic transplantation with end end anastomosis of renal arteriovenous end in rats can establish a stable and reliable animal model with fewer complications and faster healing. The success rate is similar, and the method of reserving the ligation line of renal vein during donor nephrectomy shortens the time of renal vein anastomosis.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R699.2;R-332

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