GeneXpert法检测结核分枝杆菌及其对利福平耐药性的研究

发布时间：2018-04-02 04:35

本文选题：结核分枝杆菌　切入点：GeneXpert　出处：《中华实验和临床感染病杂志(电子版)》2015年04期

【摘要】：目的探讨GeneXpert MTB/RIF(简称Xpert)法检测结核分枝杆菌及其利福平耐药性的可行性。方法选取2013年3月至6月在本院就诊的413例疑似肺结核病患者,对其痰标本分别进行抗酸染色镜检、培养、比例法体外药敏试验和Xpert法检测,并以传统的罗氏固体培养和比例法药敏试验为金标准,对Xpert法检测结果进行分析。结果以培养法为金标准,Xpert法检测197例涂阴患者的敏感性为55.2%,特异性为93.5%,与培养法检测结果差异无统计学意义(χ2=0.04,P=0.841);Xpert检测214例涂阳患者的敏感性97.5%,特异性54.5%,与培养法检测结果差异也无统计学意义(χ2=0.10,P=0.752)。以比例法药敏为金标准,对214例培养阳性的患者,Xpert法检测利福平耐药的敏感性为95.3%,特异性为99.4%,与比例法检测结果差异无统计学意义(χ2=0.00,P=1.000)。结论Xpert法适用于结核分枝杆菌及耐药结核分枝杆菌的快速筛查。
[Abstract]:Objective to investigate the feasibility of detection of Mycobacterium tuberculosis and rifampicin resistance by GeneXpert MTB RIFs.Methods 413 patients with suspected pulmonary tuberculosis were selected from March to June 2013. The sputum samples were examined by acid-fast staining, culture, in vitro susceptibility test and Xpert assay.The results of Xpert method were analyzed by using traditional Roche solid culture and proportional drug sensitivity test as gold standard.Results the sensitivity and specificity of the Xpert method for the detection of 197 smear negative patients were 55.2 and 93.55.There was no significant difference between the culture method and the culture method (蠂 2 0.04 P0. 841) Xpert was used to detect the sensitivity of 214 smear positive patients, and the specificity was 54.5%.There was no significant difference in the results (蠂 ~ 2 ~ (0.10) P ~ (0.75) ~ (2)).Using proportional drug sensitivity as gold standard, the sensitivity and specificity of Xpert method for the detection of rifampin resistance in 214 patients with positive culture were 95.3 and 99.4, respectively. There was no significant difference between the two methods (蠂 2 0.00P < 1.000).Conclusion Xpert method is suitable for rapid screening of Mycobacterium tuberculosis and resistant Mycobacterium tuberculosis.
【作者单位】：聊城市传染病医院检验科;
【基金】：山东省医药卫生科技发展计划资助(No.2009HW077)
【分类号】：R378.911

【参考文献】