兔失血性休克再灌注小A的形态学观察及α-SMA、ICAM-1的表达

发布时间：2018-04-05 13:39

本文选题：失血性休克　切入点：再灌注　出处：《新疆医科大学》2008年硕士论文

【摘要】： 目的观察新西兰大白兔失血性休克再灌注后小动脉的形态学改变,探索α-SMA、ICAM-1在再灌注后不同时点的表达与小动脉的形态改变的关系。方法将35只新西兰大白兔分为(1)对照组;(2)急性失血性休克25分钟组;(3)失血休克再灌注3/4小时组;(4)失血休克再灌注1小时组;(5)失血休克再灌注2小时组;(6)失血休克再灌注3小时组;(7)失血休克再灌注4小时组,依次分为A组、B组、C组、D组、E组、F组、G组,各组均为5只;失血休克再灌注各组在急性失血性休克25分钟后由静脉缓慢回输所抽动脉血及等量生理盐水,取心、脑、肾、空肠及下肢小动脉,用光镜和透射电镜观察小动脉内皮细胞和平滑肌细胞的形态,同时用免疫组织化学的方法显示α-SMA、ICAM-1在小动脉内皮细胞和平滑肌细胞中的表达。结果1.光镜下和电镜下见B组的小动脉内皮细胞个别脱落,平滑肌细胞轻微水肿。再灌注后内皮细胞坏死脱落、血管平滑肌细胞水肿明显,平滑肌细胞向内膜迁移,再灌注1小时损伤最显著。脑皮质与其他组织小动脉相比较,在缺血再灌注后不同时点的损伤较轻。再灌注3小时后逐渐恢复。2.A组α-SMA各组织均适度表达,B组α-SMA表达降低,再灌注1小时达到最低,再灌注3小时表达增加。D组、E组、F组、G组和A组比较,差异均有统计学意义(P0.05)。3.A组ICAM-1有少量表达,B组ICAM-1表达首先增加,再灌注后4小时达到高峰。D组、E组、F组、G组和A组比较,差异均有统计学意义(P0.05)。结论1.新西兰大白兔失血性休克再灌注小动脉内皮细胞和平滑肌细胞损伤各时间点表现不同。2.α-SMA的表达在各实验组与A组比较中有统计学差异(P0.05),提示α-SMA与血管平滑肌细胞的舒缩状态有密切的关系,平滑肌细胞在再灌注各时点处于不同的收缩状态;ICAM-1的表达在各实验组与A组比较中有统计学差异(P0.05),提示缺血再灌注具有炎症反应的特征是由于ICAM-1在内皮细胞表达升高造成的,再灌注炎症反应致使内皮细胞肿胀,小动脉内径缩小,导致血流灌注量减少。
[Abstract]:Objective to observe the morphological changes of arterioles after hemorrhagic shock reperfusion in New Zealand white rabbits and to explore the relationship between the expression of 伪 -SMA-ICAM-1 at different time points after reperfusion and the morphological changes of arterioles.3 hours after reperfusion, 7) hemorrhagic shock and 4 hours reperfusion group,The morphology of vascular endothelial cells and smooth muscle cells in jejunum and lower extremity arterioles were observed by light microscope and transmission electron microscope, and the expression of 伪 -SMA-ICAM-1 in endothelial cells and smooth muscle cells of arterioles was detected by immunohistochemical method.Result 1.Under the light microscope and electron microscope, the endothelial cells of arterioles in group B were isolated, and the smooth muscle cells were slightly edema.After reperfusion, endothelial cells necrotic and shedding, vascular smooth muscle cells edema, smooth muscle cells migrated to the intima, the most significant injury was 1 hour after reperfusion.The cerebral cortex was slightly damaged at different time points after ischemia reperfusion compared with other tissue arterioles.After 3 hours of reperfusion, the expression of 伪 -SMA in group A decreased gradually, and the expression of 伪 -SMA in group B reached the lowest level at 1 hour after reperfusion. The expression of 伪 -SMA in group D increased at 3 hours after reperfusion, and the expression of 伪 -SMA in group A was higher than that in group A, and the expression of 伪 -SMA in group A was higher than that in group A.There were significant differences in the expression of ICAM-1 in group B and group B at first, and reached the peak at 4 hours after reperfusion in group E, group E, group E, group F, group G and group A, and the difference was statistically significant (P 0.05).Conclusion 1.The expression of 伪 -SMA was significantly different in each experimental group compared with group A, suggesting that 伪 -SMA and vascular smooth muscle cells (VSMCs) were significantly different in each experimental group compared with group A, which indicated that the expression of 伪 -SMA and vascular smooth muscle cells (VSMCs) in rabbits with hemorrhagic shock and reperfusion was different at different time points.There is a close relationship between the state of relaxation and contraction,The expression of ICAM-1 in smooth muscle cells at different time points of reperfusion was significantly different between the experimental group and group A, suggesting that the inflammatory response of ischemia reperfusion was due to the increased expression of ICAM-1 in endothelial cells.The inflammatory reaction of reperfusion resulted in the swelling of endothelial cells and the reduction of arteriole diameter, which resulted in a decrease in blood perfusion.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R459.7;R361.2

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