同种异体气管原位移植模型的改良与观察

发布时间：2018-04-06 07:13

本文选题：组织工程　切入点：肺通气　出处：《中国组织工程研究》2015年20期

【摘要】：背景:气管上皮的再生能有效抑制黏膜下组织增生和管腔闭塞的发生,有研究表明通气能够加快气管上皮的再生。目的:建立并改良同种异体小鼠原位气管移植模型,进一步观察通气对供体气管的影响。方法:C57BL/6小鼠的气管作为供体,BALB/c小鼠作为受体。实验分2组,实验组取2个供体气管膜部纵行剖开,缝合成一扩大管腔的气管原位植入受体气管;对照组供体气管原位植入受体气管,28 d后获取标本进行检测。结果与结论:苏木精-伊红染色显示,与对照组相比,实验组气管管腔内上皮层可见分化良好的纤毛上皮,亦可见少量无纤毛的单层或复层扁平上皮,黏膜下轻度纤维组织增生和炎细胞浸润。形态学定量分析结果示,实验组在气管上皮中纤毛上皮的比例高于对照组(P0.05),实验组固有层与软骨的比值,固有膜纤维组织的面积、淋巴细胞浸润度均低于对照组(P0.05)。免疫组织化学染色显示,两组移植气管上皮均为为受体上皮表型。结果证实,实验成功建立了改良的小鼠原位气管移植模型,移植段气管通气量增加能够加快气管上皮分化,分化良好的气管上皮可以更好的抑制成纤维细胞的增殖。
[Abstract]:Background: the regeneration of tracheal epithelium can effectively inhibit the proliferation of submucous tissue and the occurrence of lumen occlusion. Some studies have shown that ventilation can accelerate the regeneration of tracheal epithelium.Aim: to establish and improve the orthotopic trachea transplantation model in mice and to observe the effect of ventilation on donor trachea.Methods the trachea of 10 C 57 BL / 6 mice were used as donor BALB / R c mice as receptors.The experimental group was divided into two groups. Two donor trachea membrane were opened longitudinally and sutured into an enlarged trachea implanted into the recipient trachea, while the control group obtained the specimen 28 days after the donor trachea was implanted into the recipient trachea.Results and conclusion: hematoxylin-eosin staining showed that, compared with the control group, the well-differentiated cilia epithelium could be seen in the endodermis of the trachea in the experimental group, and a small number of flat epithelium without cilia could be seen in the endodermis of the trachea.Submucosal mild fibrous tissue proliferation and inflammatory cell infiltration.The results of morphological quantitative analysis showed that the proportion of ciliated epithelium in the experimental group was higher than that in the control group (P 0.05), the ratio of lamina propria to cartilage, the area of lamina propria fibrous tissue and the degree of lymphocyte infiltration in the experimental group were lower than those in the control group (P 0.05).Immunohistochemical staining showed that the transplanted trachea epithelium in both groups were receptor epithelium phenotype.The results showed that the modified mouse model of orthotopic trachea transplantation was successfully established. The increase of trachea ventilation in the transplanted segment could accelerate the differentiation of tracheal epithelium, and the well-differentiated tracheal epithelium could better inhibit the proliferation of fibroblasts.
【作者单位】：山东省胸科医院胸外科;上海市肺科医院胸外科;上海市华东医院胸外科;
【基金】：山东省自然科学基金(ZR2010HM045)~~
【分类号】：R653;R-332

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