骨髓基质干细胞定向分化为神经细胞及其功能特征的研究

发布时间：2018-04-09 04:25

本文选题：骨髓基质干细胞　切入点：胆碱能神经元　出处：《华中科技大学》2010年硕士论文

【摘要】：目的探讨骨髓基质干细胞(BMSCs)定向分化为神经细胞的潜能及分化后细胞的功能特征。方法体外分离、扩增、纯化鼠BMSCs,取第6代细胞接种并随机分为4组:实验组A组加预诱导液(EGF+bFGF),其余各组在预诱导后加定向诱导剂(SHH+RA)时选择是否保留预诱导液分为B组(保留)和C组(去除),对照组D组不做任何干预。采用细胞免疫荧光化学法检测分化后神经样细胞的形态和功能标志。结果BMSCs在诱导剂的特定组合和时序的诱导下,各组均出现胞体收缩和突起伸出,呈现神经元细胞样改变,B组神经细胞的形态标志MAP2(72.536±1.018)%、GFAP(21.536±1.018)%明显优于其他各组,且功能标志ChAT(45.112±3.044)%、VAT(28.223±1.716)%只有B组表达。结论BMSCs在体外能定向诱导为具有一定功能的神经细胞,为脊髓损伤后细胞移植治疗奠定了基础。
[Abstract]:Objective to investigate the potential of bone marrow stromal cells (BMSCs) to differentiate into neural cells and the functional characteristics of differentiated cells.Methods isolated and amplified in vitro,BMSCs were purified, and the 6th passage cells were inoculated and randomly divided into 4 groups: group A in experimental group with EGF bFGFA, and group A in group A with directional inducer SHH RAA after preinduction, and the other groups were divided into group B (retention) and group B (retention) when adding directional inducer (SHH RAA) after preinduction.Group D did not intervene.The morphological and functional markers of differentiated nerve-like cells were detected by immunofluorescence assay.Results under the induction of specific combination of inducers and time series, BMSCs cells contracted and protruded in all groups, and the neuronal cell like changes were observed in group B (MAP2(72.536 卤1.018%), which was much better than that in other groups (21. 536 卤1. 018%), and the expression of neuronal cells in group B was significantly higher than that in group B (21. 536 卤1. 018%).The functional marker ChAT(45.112 卤3.044 was only expressed in group B (28.223 卤1.716%).Conclusion BMSCs can be induced into neural cells with certain function in vitro, which lays a foundation for cell transplantation after spinal cord injury.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R329

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