欧洲型PRRSV重组痘苗病毒候选疫苗猪体免疫效果研究

发布时间：2018-04-09 19:54

本文选题：欧洲型猪繁殖与综合征病毒　切入点：重组痘苗病毒灭活疫苗　出处：《中国病原生物学杂志》2015年08期

【摘要】：目的通过猪体免疫试验,评价欧洲型PRRSV重组痘苗病毒灭活疫苗的免疫效果。方法分别用重组痘苗病毒rVV-EU-ORF3-ORF5和灭活rVV-EU-ORF3-ORF5进行猪体免疫试验,通过检测猪血清特异性抗体中和抗体检测及T淋巴细胞增殖试验分析免疫效果。实验设E3L-VTT野毒免疫组和PBS组作对照。结果免疫后35d,欧洲型PRRSV重组痘苗病毒灭活疫苗免疫组和rVV-EU-ORF3-ORF5免疫组GP3、GP5特异抗体水平均达峰值(A值0.51~0.56),两组间比较差异无统计学意义(tGP3=1.805,tGP5=0.7998,P0.05),但均高于E3L-VTT野毒免疫组(A值0.33~0.43),差异显著(tGP3=5.518,tGP5=3.346,P0.01);猪血清中和抗体重组痘苗病毒灭活疫苗组与重组痘苗病毒疫苗组均与免疫后35d达峰值水平(分别为16.38和18.88),且两组间比较差异无统计学意义(t=0.7005,P0.05);T淋巴细胞增殖试验的SI值两疫苗组差异无统计学意义(t=0.2536,P0.05),但均高于E3L-VTT野毒免疫组(t值分比为6.414和7.222,P0.01)。结论欧洲型PRRSV重组痘苗病毒灭活疫苗能诱导猪体产生体液和细胞免疫应答,可为欧洲型PRRSV的新型疫苗的研制提供参考。
[Abstract]:Objective to pigs, evaluate the immune effect of inactivated vaccine of European type PRRSV recombinant vaccinia virus. Methods respectively and inactivated rVV-EU-ORF3-ORF5 pigs with recombinant vaccinia virus rVV-EU-ORF3-ORF5 by detecting serum immunity effect of specific antibody neutralizing antibody detection and T lymphocyte proliferation test. The E3L-VTT wild virus immune group and group PBS as control. The results of 35d after immunization, European type PRRSV recombinant vaccinia virus inactivated vaccine group and rVV-EU-ORF3-ORF5 group GP3, GP5 specific antibody level reached the peak (A = 0.51~0.56), there was no significant difference between the two groups (tGP3=1.805, tGP5=0.7998, P0.05), but were higher than that of E3L-VTT wild virus immune group (A 0.33~0.43), significant difference (tGP3=5.518, tGP5=3.346, P0.01); porcine serum neutralizing antibody of recombinant vaccinia virus inactivated vaccine group and the group of vaccinia virus vaccine groups 35d and reached the peak level after immunization (16.38 and 18.88), and there was no significant difference between the two groups (t=0.7005, P0.05); T lymphocyte proliferation test SI value of the difference between the two vaccine group had no statistical significance (t=0.2536, P0.05), but were higher than that of E3L-VTT wild virus immune group (t value as 6.414 and 7.222, P0.01). Conclusion the European type PRRSV recombinant vaccinia virus inactivated vaccine can induce porcine humoral and cellular immune responses, provide a reference to develop new vaccines for European type PRRSV.

【作者单位】：中国人民解放军军事医学科学院军事兽医研究所;吉林大学动物医学学院;吉林农业大学动物科技学院;
【基金】：科技部863项目(No.2011AA10A208)
【分类号】：R392

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