改良聚肌胞在人用狂犬病疫苗中的佐剂作用

发布时间：2018-04-11 09:00

本文选题：狂犬病疫苗 + 佐剂　；参考：《沈阳药科大学》2008年硕士论文

【摘要】： 狂犬病是传染病中病死率最高的疾病之一,严重威胁着人们的生命安全。目前,接种狂犬病疫苗是预防狂犬病发生的最有效方法之一。但单纯注射疫苗预防狂犬病往往达不到理想效果。因此,研制更加安全、高效的狂犬病疫苗是药学界研究的焦点。本研究采用切向流过滤技术,通过100K分子量的超滤膜包将市售聚肌胞注射液进行超滤浓缩,去除小分子聚肌胞。根据检测含量调整聚肌胞浓度为2mg/ml,并对其进行分子量大小、增色效应检测。经过改良后的聚肌胞注疫苗佐剂分子量在30万—55万之间,增色效应符合要求。利用改良后浓度为2mg/ml的聚肌胞与原市售聚肌胞注射液作为冻干疫苗稀释液分别复溶冻干人用狂犬病疫苗。对复溶后的狂犬病疫苗进行了检测,结果表明:疫苗效价对比提高了1.4～2.3个单位,诱生小白鼠血清干扰素水平提高了6倍以上,明显地提高了细胞免疫功能及小白鼠中和抗体水平,无豚鼠全身主动过敏反应。初步确认了改良后聚肌胞的佐剂作用。
[Abstract]:Rabies is one of the diseases with the highest mortality among infectious diseases, which is a serious threat to the safety of people.At present, rabies vaccination is one of the most effective methods to prevent rabies.But the simple injection vaccine often can not achieve the ideal effect to prevent rabies.Therefore, the development of a safer and more efficient rabies vaccine is the focus of pharmaceutical research.In this study, a tangential flow filtration technique was used to remove small molecule polymyocytes by ultrafiltration membrane package with molecular weight of 100K to concentrate the marketable polymyocyst injection.The concentration of polymyocytes was adjusted to 2 mg / ml according to the detection content, and the molecular weight and chromaticity effect were detected.The molecular weight of the modified polymyocyte-injected vaccine adjuvant was between 300000 and 550000.The modified 2mg/ml polymyocytes and the original marketable polymyocyst injection were used as the diluent of freeze-dried vaccine for lyophilized human rabies vaccine respectively.The recombinant rabies vaccine was tested. The results showed that the titer of rabies vaccine was increased by 1.4 ~ 2.3 units, and the serum interferon level of induced mice was increased by more than 6 times.The cellular immune function and neutralizing antibody level in mice were significantly improved, and there was no systemic active allergic reaction in guinea pigs.The adjuvant effect of modified polymyocytes was confirmed.
【学位授予单位】：沈阳药科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

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