人羊膜上皮细胞与人羊膜间充质干细胞生物学特性对比

发布时间：2018-04-11 11:39

本文选题：人羊膜上皮细胞 + 人羊膜间充质干细胞　；参考：《中国实验血液学杂志》2015年04期

【摘要】：目的:比较人羊膜上皮细胞(human amnion epithelial cells,h AECs)与人羊膜间充质干细胞(human amnion mesenchymal stem cells,h AM SCs)的生物学特性。方法:从足月剖宫产胎盘上剥离羊膜组织,采用低速胰蛋白酶-胶原酶消化法,分别分离获取h AECs及h AMSCs,观察此两种细胞的增殖生长情况,采用免疫荧光染色、流式细胞术、逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)、诱导分化染色等方法对两种细胞进行生物学特性鉴定及对比。结果:h AECs为圆形或椭圆形,呈铺路石样生长,最多传至5代;h AMSCs为成纤维细胞样的长梭形,呈放射状或漩涡状分布生长,可传至30代左右。h AECs表达上皮细胞标志角蛋白19(cytokeratin19,CK19),部分表达波形蛋白;h AM SCs表达波形蛋白,不表达CK19。两种细胞均表达CD29、CD44、CD73、CD90、OCT-4、Nanog。h AECs不表达CD105,成骨及成脂诱导阳性。结论:h AECs和h AM SCs均具有某些干细胞特性,h AECs不能无限增殖,其生长增殖及分化能力均低于h AM SCs。
[Abstract]:Aim: to compare the biological characteristics of human amnion epithelial cells and human amnion mesenchymal stem cells with human amniotic mesenchymal stem cells (AECs).Methods: amniotic membrane tissue was removed from the placenta of term cesarean section. H AECs and h AMSC were isolated and obtained by low speed trypsin collagenase digestion. The proliferation and growth of these two kinds of cells were observed by immunofluorescence staining and flow cytometry.Reverse transcriptase polymerase chain reaction (RT-PCR) reverse transcription-polymerase chain reactionation assay (RT-PCR) and induced differentiation staining were used to identify and compare the biological characteristics of the two kinds of cells.Results the AECs was round or elliptical, and grew like paving stone. At most, the fifth generation of AMSCs was long fusiform like fibroblast, and distributed in radial or whirlpool shape.The expression of keratin 19 cytokeratin19CK19 and vimentin hAM SCs were expressed at about 30 passages, but not CK19.Both kinds of cells expressed CD29, CD44, CD73, CD90, OCT-4 and Nanog.h AECs, and were positive for osteogenesis and adipogenic induction.Conclusion both AECs and hAM SCs have some stem cell characteristics. H AECs can not proliferate indefinitely, and its ability of growth, proliferation and differentiation is lower than that of hAM SCs.
【作者单位】：广州市妇女儿童医疗中心;
【分类号】：R329.2

【参考文献】