糖代谢异常对大鼠肝脏组织中巨噬细胞移动抑制因子及C-Jun氨基端激酶表达水平的影响

发布时间：2018-04-15 14:23

本文选题：巨噬细胞移动抑制因子 + C-Jun氨基端激酶　；参考：《中国实验动物学报》2015年02期

【摘要】：目的观察巨噬细胞移动抑制因子(MIF)和C-Jun氨基端激酶(JNK)在糖代谢异常大鼠肝脏组织中表达水平的变化,探讨糖代谢异常合并非酒精性脂肪肝(NAFLD)的病理机制。方法将60只大鼠随机分为糖耐量受损(IGT)模型组(n=20)、2型糖尿病(T2DM)模型组(n=20)、IGT对照组(n=10)及T2DM对照组(n=10),高脂饲料喂养复制IGT大鼠模型,高脂饲料喂养加腹腔注射小剂量链脲佐菌素(STZ)制备T2DM大鼠模型,采用TUNEL法检测各组大鼠肝脏细胞凋亡;实时荧光PCR技术检测肝脏组织中MIF mRNA的表达;Western blot方法检测肝脏组织中MIF、caspase-3、JNK蛋白表达及磷酸化JNK(p-JNK)的表达。结果 IGT大鼠及T2DM大鼠肝组织凋亡细胞明显增多;IGT组和T2DM组肝组织MIF基因表达较各自对照组明升高(P0.01),MIF、caspase-3、JNK蛋白表达及JNK磷酸化水平也明显升高(P0.05或P0.01);与IGT组相比,T2DM组caspase-3、MIF、JNK蛋白表达水平明显降低(P0.01),而JNK磷酸化水平是明显升高(P0.01)。结论糖代谢异常合并非酒精性脂肪肝的发生可能与MIF、caspase-3、JNK表达水平的升高及JNK磷酸化水平的增强有关。
[Abstract]:Objective to observe the expression of macrophage migration suppressor (MIF) and C-Jun aminoterminal kinase (C-Jun) in the liver of rats with abnormal glucose metabolism and to explore the pathological mechanism of abnormal glucose metabolism combined with NAF LDD.Methods Sixty rats were randomly divided into two groups: the model group with impaired glucose tolerance (IGT) group and the model group with type 2 diabetes mellitus (T2DMM). The control group was divided into two groups: the control group (n = 10) and the T2DM control group (n = 10). The IGT model was induced by high fat diet feeding.The rat model of T2DM was established by high fat feed and intraperitoneal injection of streptozotocin (STZ). The apoptosis of liver cells in each group was detected by TUNEL method.The expression of MIF mRNA in liver tissue was detected by real-time fluorescence PCR. The expression of MIF- caspase-3 PCR protein and phosphorylated JNK-p-JNK-JNK protein in liver tissue were detected by Western blot.Results the expression of MIF gene in liver tissue of IGT group and T2DM group was significantly higher than that of control group. The expression of MIF protein and the level of JNK phosphorylation were also significantly increased in IGT group and T2DM group, compared with IGT group, the expression of MIF gene in T2DM group was significantly higher than that in IGT group.The expression level of JNK protein in caspase-3 was significantly decreased, while the level of JNK phosphorylation was significantly increased.Conclusion the abnormal glucose metabolism and the occurrence of non-alcoholic fatty liver may be related to the increase of JNK phosphorylation and the increase of JNK phosphorylation.
【作者单位】：山西医科大学实验动物中心实验动物和人类疾病动物模型山西省重点实验室;山西省一0九医院;山西医科大学第二医院肿瘤生物治疗科;
【基金】：山西省实验动物专项资金资助项目(2013k11) 山西医科大学科技创新基金(01201425)
【分类号】：R363

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