大肠埃希菌中16S rRNA甲基化酶armA基因的分布与耐药性的研究

发布时间：2018-04-17 02:03

本文选题：大肠埃希菌 + S　；参考：《中国卫生检验杂志》2015年01期

【摘要】：目的调查本院大肠埃希菌arm A基因的分布定位与耐药谱的关系。方法对氨基糖苷类抗生素耐药的大肠埃希菌检测arm A和intl1。采用质粒接合试验初步定位,基因间重复序列-聚合酶链式反应(ERIC-PCR)进行基因分型。结果 69株大肠埃希菌中共有arm A基因阳性菌株20株,均为ESBL阳性。含有arm A基因的大肠埃希菌intl1阳性率为60.0%,含有5种不同的耐药基因盒,分别携带drf A25、drf A1、drf A12、aad A1、aad A2、sat和blaVEB-1基因。ERICPCR显示arm A阳性大肠埃希菌分为5型,A型为优势克隆株。质粒接合试验发现A克隆株获得阿米卡星耐药接合子,而B、C克隆株均未获得;质粒谱分析发现A克隆株的arm A基因均位于23 130 bp质粒上。结论 arm A基因广泛存在于大肠埃希菌中,且呈克隆播散。A克隆株具有质粒传播能力,应采取有效措施控制耐药菌的传播。
[Abstract]:Objective to investigate the relationship between arm A gene distribution and drug resistance spectrum of Escherichia coli in our hospital.Methods arm A and intl1 were detected by Escherichia coli resistant to aminoglycoside antibiotics.The plasmids conjugation test was used to localize the genotypes of ERIC-PCR- ERIC-PCR.Results there were 20 arm A positive strains in 69 Escherichia coli strains, all of which were ESBL positive.The positive rate of intl1 of Escherichia coli containing arm A gene was 60.0.The positive rate of Escherichia coli was 60.0.The positive Escherichia coli containing five different drug resistant gene cassettes, carrying drf A25drf A1drf A12aad A1A2A2Ssat and blaVEB-1 gene .ERICPCR, showed that arm A positive Escherichia coli could be divided into 5 types A as dominant clone strains.Plasmid conjugation test showed that A clone obtained amikacin resistant conjugate, but none of BHG C clone obtained Amikacin resistant conjugate, and plasmid profile analysis showed that the arm A gene of A clone was all located on 23 130bp plasmid.Conclusion arm A gene is widely present in Escherichia coli and has the ability of plasmid transmission. Effective measures should be taken to control the transmission of drug-resistant bacteria.
【作者单位】：绍兴市中医院;绍兴市人民医院临床检验中心;绍兴市人民医院病理科;
【基金】：浙江省医学重点学科建设计划项目(GJSX-010-004)
【分类号】：R378.21

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