SD大鼠坐骨神经内微血管定量分析模型

发布时间：2018-04-25 15:56

本文选题：显微外科 + 周围神经　；参考：《中国科技论文》2015年24期

【摘要】：比较氧化铅和伊凡斯兰(evans blue,EB)灌注后血管定量研究的优劣,寻找最适的微血管定量分析模型。取15只健康成年SD大鼠,随机分为荧光显影组A(EB一维组)、荧光显影组B(EB三维组)和放射造影组C,分别灌注单纯EB、明胶EB和明胶氧化铅后,对各组使用紫外分管光度计、体式显微镜、反射荧光显微镜和micro-CT进行观察、测量并计算血管直径(D)、血管指数(SF)和血管容积(VF)。结果表明:A组可测得局部血容量为(16.22±1.70)mm3,B组的血管直径、血管面积和血管指数分别为(11.79±7.23)μm、(0.14±0.05)mm2和(24.19±5.03)%;而C组分别为(26.45±11.81)μm、(0.06±0.02)mm2和(10.73±2.06)%。荧光B组对直径20μm微血管的显影效果较放射组好(P0.01),但对直径20μm微血管的显影效果无显著性差异(P0.05)。EB三维研究优于EB一维法,不仅能够显示微血管形态,而且能够采集数据进行定量分析,但其不利于大样本研究。明胶氧化铅灌注易于操作,虽然分辨率较EB显影低,但更适用于大样本可视定量研究。
[Abstract]:Objective: to compare the advantages and disadvantages of vascular quantitative study after perfusion with lead oxide and Ivanovanevans blueEBs, and to find the most suitable microvascular quantitative analysis model. Fifteen healthy adult Sprague-Dawley rats were randomly divided into one dimensional A(EB group and three dimensional fluoroscopy group (B(EB 3D group) and radiography group (C). After infusing pure EBB, gelatin EB and lead gelatin oxide respectively, ultraviolet tube photometer was used in each group. The vessel diameters, vascular index (SF) and vascular volume (VF) were measured and calculated by volume microscope, reflectance fluorescence microscope and micro-CT. The results showed that the local blood volume in group A was 16.22 卤1.70 mm ~ 3mm ~ (-3), the diameter, area and index of blood vessels were 11.79 卤7.23 渭 m ~ (0.14 卤0.05)mm2) and 24.19 卤5.03 ~ (th), respectively, while those in group C were 26.45 卤11.81 渭 m 卤0.06 卤0.02)mm2 and 10.73 卤2.06A, respectively. The development effect of 20 渭 m microvessel in Group B was better than that in Group B, but there was no significant difference in the development effect of Group B with diameter of 20 渭 m microvessel. The three-dimensional study of P0.05? EB was superior to that of one dimensional method of EB, which could not only show the morphology of microvessel. And can collect data for quantitative analysis, but it is not conducive to large sample research. Gelatin lead oxide perfusion is easy to operate and is more suitable for large sample visual quantitative research although its resolution is lower than that of EB.
【作者单位】：中山大学附属第一医院;温州医学院基础医学院;
【基金】：国家高技术研究发展计划(863计划)资助项目(2006AA02A130) 高等学校博士学科点专项科研基金资助项目(20120171120075)
【分类号】：R-332

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