分离自婴儿粪便的嗜酸乳杆菌和长双歧杆菌的常温保存探究

发布时间：2018-04-28 22:10

本文选题：嗜酸乳杆菌 + 长双歧杆菌　；参考：《生物技术通讯》2015年06期

【摘要】：目的:试验不同的保护剂成分配方,探究分离自婴儿粪便的2种益生菌(嗜酸乳杆菌和长双歧杆菌)在常温条件下保持较高活性的可行方法。方法:利用均匀设计实验方法设计保护剂配方,用于保存特定高活性高稳定性的菌种,在不同时间点检测活菌数,分析数据得出最佳保护剂成分比例。结果:嗜酸乳杆菌10A31保护剂最佳配方为:海藻糖∶谷氨酸∶天冬氨酸=5∶24∶24;长双歧杆菌11A11保护剂最佳配方为:海藻糖∶谷氨酸∶麦芽糖=34∶11∶10;用最佳保护剂配方保存后,嗜酸乳杆菌10A31在200 d时的菌落计数结果为3.87×109CFU/g,长双歧杆菌11A11在160 d时为1.53×109CFU/g,均具有较高的活性。结论:最佳保护剂配方使2个菌种常温保存期至160 d。
[Abstract]:Objective: to test different formulations of protective agents and to explore a feasible method of keeping high activity of two probiotics (Lactobacillus acidophilus and bifidobacterium long) isolated from infant faeces at room temperature. Methods: the formula of protective agent was designed by uniform design method, which was used to preserve the specific bacteria with high activity and high stability. The number of live bacteria was detected at different time points, and the optimum proportion of protective agent components was obtained by analyzing the data. Results: the best formula of 10A31 protective agent for Lactobacillus acidophilus was trehalose: glutamic acid: aspartic acid 5: 24: 24; 11A11 protection agent for bifidobacterium longifera was trehalose: glutamic acid: maltose 34: 11: 10; After the preservative formula is preserved, The colony count of Lactobacillus acidophilus 10A31 was 3.87 脳 10 9 CFU / g at 200d, and 11A11 of Bifidobacterium was 1.53 脳 10 9 CFU / g at 160d. Conclusion: the best formula of protective agent can keep the two strains for 160 days at room temperature.
【作者单位】：华南农业大学生命科学学院广东省农业生物蛋白质功能与调控重点实验室;广州市微生物研究所;
【基金】：广东省自然科学基金(2014A030313449) 广州市科技惠民项目(2014Y2-00116) 广州市科学研究项目(201510010057)
【分类号】：R371

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