RAC1基因多态性对其mRNA转录和表达的影响

发布时间：2018-04-30 07:58

本文选题：Rho + GTP　；参考：《中国医院药学杂志》2015年23期

【摘要】：目的:探讨RAC1基因上4个单核苷酸多态性(SNPs)对其mRNA转录和表达的影响,为进一步研究RAC1基因多态性与相关疾病及药物反应性之间的关系做铺垫。方法:实时荧光TaqMan-MGB探针等位基因分型技术被用来测定242例湖北地区健康志愿者的RAC1基因上4个SNPs位点的基因型,同时采用Trizol法提取总mRNA并用RTQ-PCR进行定量分析。结果:本研究所选4个SNPs位点均符合Hardy-Weinberg平衡,且各SNP位点相互独立。4个SNPs的等位基因、基因型分布频率在不同性别间没有差异(P0.05)。RAC1基因转录的mRNA的表达量与性别和年龄不相关(P0.05)。携带rs702482-TT基因型者的RAC1 mRNA比携带其他两种基因型的人RAC1 mRNA表达量低,且差异有统计学意义(P0.05)。结论:RAC1基因转录的mRNA的表达量与性别和年龄无关(P0.05),但受rs702482-TT基因型的影响而表达下调。
[Abstract]:Objective: to investigate the effects of four single nucleotide polymorphisms (SNPs) on the transcription and expression of RAC1 gene mRNA in order to further study the relationship between RAC1 gene polymorphism and related diseases and drug reactivity. Methods: the allelic typing technique of real-time fluorescent TaqMan-MGB probe was used to determine the genotypes of 4 SNPs loci of RAC1 gene in 242 healthy volunteers from Hubei province. The total mRNA was extracted by Trizol method and analyzed quantitatively by RTQ-PCR. Results: the four SNPs loci were all in line with the Hardy-Weinberg equilibrium, and each SNP locus was independent of each other. There was no difference in the frequency of genotypic distribution of 4 SNPs alleles between genders and the amount of mRNA transcripted by P0.05. RAC1 gene was not correlated with sex and age (P0.05). The RAC1 mRNA expression in the rs702482-TT genotype was lower than that in the other two genotypes, and the difference was statistically significant (P 0.05). Conclusion the expression of mRNA in the mRNA transcription of the RAC1 gene is not related to sex and age, but it is down-regulated by the rs702482-TT genotype.
【作者单位】：华中科技大学同济医学院附属协和医院药剂科;
【基金】：国家自然科学基金项目(编号:81273591) 湖北省自然科学基金资助项目(编号:2009CDB380) 中央高校基本科研业务费专项资金资助(编号:2014YGYL003)
【分类号】：R3416

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