几种体外抗氧化检测方法的评价研究

发布时间：2018-04-30 20:31

本文选题：抗氧化方法评价 + 相关性分析　；参考：《陕西师范大学》2010年硕士论文

【摘要】： 体外抗氧化是抗氧化物质评价的重要方法,由于体外抗氧化检测体系简单,操作迅速,成本低,使得体外抗氧化评价方法在食品、药品等抗氧化效应的评价方面使用日益广泛,但是由于体外抗氧化检测体系大多为纯化学反应体系,不同检测反应体系中所使用的自由基种类、自由基产生方法、自由基损伤的底物种类、损伤检测原理等各不相同,导致不同的抗氧化检测方法所获得的结果相关性较差,不同抗氧化检测方法对同一种抗氧化剂进行评价可以获得不同的结果,甚至相互矛盾,利用不同抗氧化检测方法对不同抗氧化剂进行评价,对抗氧化剂抗氧化能力强弱的判定往往差别较大,使得研究者难以给出确切的结论。抗氧化效应的检测目的在于筛选可以用于清除人体内自由基的抗氧化物质,用于疾病治疗或者预防的目的,所以体外的抗氧化检测方法需要尽可能吻合体内的状况,才有可能真实的评价抗氧化物质的抗氧化效果,很多体外抗氧化方法采用的是纯化学反应体系,所用的自由基种类和生理自由基不一致,检测自由基损伤的分子种类与生物体内的损伤物质也不对应,使得体外检测所得的抗氧化物质在体实验往往效果不明显。我们以常见的四种抗氧化剂为试验样品,利用不同的抗氧化检测方法对其抗氧化效果进行评估,根据评估的结果进行相关性分析,将相关性高的方法进行归类,并依据其反应体系的特点对其进行初步分析评价,为研究者选择合适的体外抗氧化方法提供科学依据。通过对实验结果的分析发现：第一,结晶紫法、2-脱氧-D-核糖法、TBA法,这位三种方法之间相关性较高,在抗氧化物质的能力检测中,这三种方法应该一起选择,得到的结果可能代表抗氧化剂对羟基自由基的清除能力或者脂质的保护能力。第二,肝脏线粒体脂质过氧化检测分别与结晶紫法、TBA法、抑制蛋白质羰基化检测、双氧水诱导红细胞溶血法有很好的结果相关性,是更适合生物体内情况的抗氧化检测方法,可以用于检测物质对生物膜系统的保护能力,或者对于脂质,蛋白质的保护能力。第三,清除DPPH自由基法、亚硝基R盐钴离子褪色法、邻苯三酚自氧化法,这3种方法也是有较高的结果相关性的,可以用来检测抗氧化剂对多数自由基的清除能力。
[Abstract]:Antioxidation in vitro is an important method for the evaluation of antioxidant substances. Because of its simplicity, rapid operation and low cost, in vitro antioxidant evaluation methods are increasingly used in the evaluation of antioxidant effects such as food, drugs and so on. However, the antioxidant detection systems in vitro are mostly pure chemical reaction systems. The kinds of free radicals used in different detection reaction systems, the methods of free radical generation, the types of free radical damage substrate, the principle of damage detection and so on are different. The results obtained by different antioxidant detection methods are poor, and different antioxidant detection methods can obtain different results, even contradictory results, when evaluating the same antioxidant. Using different antioxidant detection methods to evaluate different antioxidants, the determination of antioxidant capacity is often different, which makes it difficult for researchers to draw a definite conclusion. The purpose of antioxidant effect detection is to screen antioxidants that can be used to scavenge free radicals in human body, for disease treatment or prevention purposes, so in vitro antioxidant detection methods need to be as consistent as possible in vivo. It is possible to truly evaluate the antioxidant effect of antioxidants. Many methods of antioxidation in vitro use pure chemical reaction systems, and the kinds of free radicals used are inconsistent with physiological free radicals. The molecular types of free radical damage do not correspond to the damage substances in vivo, so the effect of antioxidants detected in vitro is not obvious. Four common antioxidants were used as test samples to evaluate the antioxidative effect by using different antioxidant detection methods. According to the results of the evaluation, the correlation analysis was carried out, and the highly correlated methods were classified. According to the characteristics of its reaction system, it was preliminarily analyzed and evaluated to provide scientific basis for the selection of suitable antioxidant methods in vitro. Through the analysis of the experimental results, it is found that: first, the crystal violet method and 2-deoxy--Dribose method TBA method, these three methods have a high correlation, in the ability of antioxidant substances, the three methods should be selected together. The results may indicate the ability of antioxidants to scavenge hydroxyl radicals or protect lipids. Second, the detection of liver mitochondrial lipid peroxidation has a good correlation with crystal violet method, inhibition of protein carbonylation, and hydrogen peroxide induced erythrocyte hemolysis. It is a more suitable antioxidant detection method in vivo. Can be used to test the protection of substances to the biofilm system, or to protect lipids, proteins. Third, scavenging DPPH free radical method, cobalt ion fading method of nitroso R salt and pyrogallol autooxidation method also have a high correlation with the results, which can be used to detect the scavenging ability of antioxidants to most free radicals.
【学位授予单位】：陕西师范大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R346

【引证文献】